摘要
以含有广谱稻瘟病抗性基因Pi-1的籼稻材料BL122为供体,优质感病温敏核不育系GD-8S为受体,通过杂交、多次回交和自交结合分子标记辅助选择技术,将Pi-1基因导入GD-8S中。综合目标基因的分子检测、育性镜检、田间农艺性状表现以及遗传背景的回复率,筛选获得5个改良株系RGD8S-1、RGD8S-2、RGD8S-3、RGD8S-4和RGD8S-5。采用广东省具有代表性的33个菌株进行人工接种抗性鉴定,5个改良株系的抗性频率为75.76%～100.00%,而对照仅为9.09%。农艺性状分析表明,除剑叶长和每株穗数外,其余性状改良株系与对照无显著差异。这些改良株系可用于进一步测配选育高抗稻瘟病杂交稻新组合。
The broad-spectrum blast resistance gene Pi-1, from the donor line BL122, was introduced into a /hermosensitive genic male sterile （TGMS） line GD-8S, which possessed good grain quality but high susceptibility to rice blast, by using backcross breeding and molecular marker-assisted selection. Five elite improved male sterile lines RGD8S-1, RGD8S-2, RGD8S-3, RGD8S-4 and RGD8S-5, were selected based on the results of molecular marker analysis, spikelet sterility, recovery ratio of genetic background and agronomic traits. Thirty-three representative blast isolates collected from Guangdong Province, China were used to inoculate the improved lines and the origin line GD-8S artificially. The resistant frequency of the improved lines ranged from 75.76% to 100.00%, much higher than that of the check GD-SS, which was only 9.09%. On the agronomic characters, there was no significant differences between the improved lines and the check GD-8S except for flag leaf length and panicle number per plant. The improved lines could be used for breeding hybrid rice with high blast resistance.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2007年第6期599-604,共6页
Chinese Journal of Rice Science
基金
国家863计划资助项目(2001AA2410111
2003AA212050)
广东省科技重大专项资助项目(2003A2010101
2006A20202001)
广东省农业攻关资助项目(2005B20101006)
关键词
稻瘟病抗性
温敏核不育系
分子标记辅助选择
育种
水稻
blast resistance
thermo-sensitive genic male sterile line
molecular marker-assisted selection
breedingl rice
