摘要
通过对Pib抗、感稻瘟病基因型序列进行比对,开发分子标记,并搜集已报道的8个抗稻瘟病基因(Pi9、Pi-k^(h)、Pit、Pi5、Pita、Pb1、Pi2、Pikm)的分子标记,分析这9个抗稻瘟病基因在291份水稻种质资源(以黄淮海区域种质为主)中的分布及组合特征,为今后抗稻瘟病分子标记辅助选择育种提供理论依据。结果表明,成功开发了Pib基因分子标记。单份水稻种质资源中携带的抗稻瘟病基因数目为0~6个,携带3个抗稻瘟病基因的种质资源最多,占比40.9%,其次为携带2个(20.3%)和4个(19.2%)抗稻瘟病基因的种质资源,携带6个抗稻瘟病基因的种质资源最少(0.4%)。9个抗稻瘟病基因在水稻种质资源中出现的频率存在较大差异,介于7.2%~59.1%,Pi9基因出现频率最高(59.1%),Pi-k^(h)次之(50.8%),随后依次为Pib(41.2%)、Pi5(36.4%)、Pita(29.2%)、Pb1(28.8%)、Pi2(19.5%)、Pikm(17.1%),Pit基因出现频率最低(7.2%)。291份水稻种质资源中共有抗稻瘟病基因组合93个,Pi9+Pi-k^(h)+Pita组合水稻种质资源最多(18份),其次为Pi9+Pi-k^(h)+Pib组合(17份)和Pi9+Pb1组合(11份),Pi9+Pi5+Pb1、Pi9+Pi-k^(h)和Pik^(h)+Pita组合均有9份,其他组合均低于8份。
The molecular markers of eight blast resistant genes(Pi9,Pi-k^(h),Pit,Pi5,Pita,Pb1,Pi2 and Pikm)were collected,and the Pib molecular marker was developed by comparing the sequences of rice blast resistant and susceptible genotypes of Pib.Based on these molecular markers,we analyzed the distribution and combination features of these nine rice blast resistant genes in 291 rice germplasm resources(mainly from Huang-Huai-Hai region),so as to provide a theoretical basis for molecular marker assisted selection breeding for rice blast resistance in the future.The results showed that the molecular marker of Pib gene was successfully developed.The number of rice blast resistance genes in a rice germplasm resource was 0—6,and the number of germplasm resources carrying three rice blast resistance genes was the most,accounting for 40.9%,followed by the number of germplasm resources carrying two(20.3%)and four(19.2%)rice blast resistance genes,and the germplasm resources carrying six rice blast resistance genes were the least(0.4%).The frequency of nine rice blast resistance genes in rice germplasm resources was obviously different,ranging from 7.2%to 59.1%.The frequency of Pi9 gene was the highest(59.1%),followed by Pi-k^(h)(50.8%),Pib(41.2%),Pi5(36.4%),Pita(29.2%),Pb1(28.8%),Pi2(19.5%)and Pikm(17.1%),and the frequency of Pit gene was the lowest(7.2%).There were 93 rice blast resistance gene combinations in 291 rice germplasm resources.Pi9+Pi-k^(h)+Pita combination number was the most in rice germplasm resources(18),followed by Pi9+Pi-k^(h)+Pib combination(17),Pi9+Pb1 combination(11).The number of Pi9+Pi5+Pb1,Pi9+Pi-k^(h)and Pi-k^(h)+Pita combinations was the same(9),and the number of the other combinations was less than eight.
作者
桑世飞
王亚男
王君怡
曹梦雨
孙晓涵
姬生栋
SANG Shifei;WANG Ya'nan;WANG Junyi;CAO Mengyu;SUN Xiaohan;JI Shengdong(College of Life Sciences,Henan Normal University,Xinxiang 453007,China;Henan Crop Genetic Improvement and Germplasm Innovation Engineering Research Center,Xinxiang 453007,China)
出处
《河南农业科学》
北大核心
2022年第12期19-27,共9页
Journal of Henan Agricultural Sciences
基金
河南省科技攻关计划项目(212102110064)
河南水稻产业体系项目(Z2012-04-G02)
国家重点研发计划项目(2018YFD0200200)。
关键词
水稻
抗稻瘟病基因
分子标记
基因组合
PIB
Rice
Rice blast resistant gene
Molecular marker
Gene combination
Pib
