摘要
开发适用于西瓜种质基因分型的KASP(kompetitive allele specific PCR)标记,并构建种质指纹图谱,对促进西瓜种质资源的保护、品种鉴定和遗传改良具有重要的科学意义。根据西瓜重测序数据分析鉴定的高质量核苷酸多态性(single nucleotide polymorphism,SNP)位点,共设计50个KASP标记,并在43份西瓜材料中进行基因分型,进一步通过聚类分析和指纹图谱构建,评估其遗传多样性。结果表明:KASP标记设计的成功率为40.0%,但不同染色体上KASP标记成功率有所差异,其中1号和7号染色体的成功率最高,达到75.0%;挑选16个最优KASP标记,根据其在43份西瓜材料中的基因分型结果构建指纹图谱,将其转化为二维码,便于快速识别和查询西瓜材料遗传信息;对43份材料进行聚类分析,发现饲用西瓜大都形成了一个独立的分支,且遗传距离相近,而黏籽西瓜和栽培西瓜则在多个分支中分布,遗传距离相对较远,充分显示出它们之间遗传的多样性和复杂性。试验成功开发并应用西瓜KASP标记技术,为西瓜遗传研究与种质资源评价提供了新的工具和方法,也为西瓜的品种鉴定和种质资源管理提供了科学依据。
This study aimed to develop kompetitive allele specific PCR(KASP)markers suitable for genotyping and fingerprint map construction of watermelon germplasms,so as to enhance the protection of watermelon germplasm resources,variety identification,and genetic improvement.Based on the high-quality single nucleotide polymorphism sites(SNPs)identified through the re-sequencing data analysis of watermelon,50 KASP markers were designed and employed for genotyping 43 watermelon accessions.Genetic diversity was assessed via cluster analysis and fingerprinting.The overall success rate of KASP marker design was 40.0%,with significant variations across chromosomes.Notably,chromosomes 1 and 7 had the highest success rate of 75.0%.Sixteen optimal KASP markers were selected for genotyping the 43 watermelon accessions.Their genotyping results were used to establish standardized fingerprint maps,which were then encoded into two-dimensional codes.This allows for quick retrieval of genetic information and identification of watermelon materials.Cluster analysis revealed that watermelon accessions from Citrullus amarus predominantly formed a distinct clade with close genetic relationship.In contrast,accessions from Citrullus mucosospermus and Citrullus lanatus were broadly distributed across multiple branches,indicating significant genetic divergence and complexity.This research has successfully established and applied KASP technology for watermelon,offering a new tool for watermelon genetic research and germplasm resource evaluation.It also provides a scientific basis for watermelon cultivar identification and germplasm management.
作者
张跃星
张瑞青
肖玉珍
代雪
张勇
王中元
于蓉
侯尹婕
张显
魏春华
ZHANG Yuexing;ZHANG Ruiqing;XIAO Yuzhen;DAI Xue;ZHANG Yong;WANG Zhongyuan;YU Rong;HOU Yinjie;ZHANG Xian;WEI Chunhua(College of Horticulture,Northwest A&F University,Yangling 712100,Shaanxi,China;Institute of Horticulture,NingxiaAcademy of Agriculture and Forestry Sciences,Yinchuan 750000,Ningxia,China)
出处
《中国蔬菜》
北大核心
2025年第8期54-64,共11页
China Vegetables
基金
2023年西甜瓜良种联合攻关项目,宁夏农林科学院高质量发展和生态保护科技创新示范项目(NGSB-2021-7)。
