摘要
为了明确不同类型嫁接砧木对设施西瓜品质的影响,筛选适宜宁夏设施栽培西瓜的嫁接砧木,以宁美2号(NM2)西瓜为接穗,宁砧2号(南瓜,NZ2)、强根(葫芦,QG)、宁砧3号(野生西瓜,NZ3)为砧木,通过研究嫁接后西瓜品质变化,结合转录组学和代谢组学分析,比较3种类型砧木对设施西瓜品质的影响。结果表明,不同类型砧木嫁接后显著影响设施西瓜的基因表达、生理生化特性和果实品质。其中,NZ2嫁接后西瓜中心可溶性固形物含量增加了0.2个百分点,维生素C含量提高了2.7%;同时也影响了蛋白质加工通路,可能通过增强天然防御机制改善品质特性。QG嫁接后对西瓜的基因表达和代谢物变化影响最显著,但果实品质略差。NZ3嫁接后西瓜的粗纤维含量最高,比NM2高65.79%。通过转录组和代谢组技术研究嫁接后果实品质变化,为科学筛选砧木和提高嫁接西瓜品质提供了理论依据。通过综合分析,南瓜型砧木NZ2可作为适宜的砧木品种。
To clarify the effects of different types of rootstocks on the quality of watermelon cultivated in greenhouse and screen suitable rootstocks for watermelon cultivation in Ningxia facilities,Ningmei 2(NM2)watermelon was used as scion,and Ningzhen 2(pumpkin,NZ2),Qianggen(gourd,QG),Ningzhen 3(wild watermelon,NZ)as rootstocks,to investigate post-grafting changes in watermelon quality.By combining transcriptomic and metabolomic analyses,the influence of the three rootstock types on watermelon quality was compared.The results showed that different rootstock types significantly affected gene expression,physiological and biochemical properties,and fruit quality of watermelon cultivated in greenhouse.Among them,NZ2 grafting increased the central soluble solids content by 0.2 percentage points,and the vitamin C content by 2.7%.It also significantly influenced protein processing pathways,potentially enhancing quality by bolstering natural defense mechanisms.QG grafting had the most significant influence on gene expression and metabolite changes but resulted in slightly inferior fruit quality.NZ3 grafted watermelon exhibited the highest crude fiber content,65.79%higher than that of NM2.The application of transcriptomic and metabolomic techniques to study the changes of fruit quality after grafting provides a theoretical basis for scientific screening of rootstocks and improving the quality of grafted watermelon.Through comprehensive analysis,the pumpkin rootstock NZ2 was considered as a suitable rootstock variety.
作者
杨万邦
王晓媛
于蓉
郭松
岳贞
杜慧莹
田梅
YANG Wanbang;WANG Xiaoyuan;YU Rong;GUO Song;YUE Zhen;DU Huiying;TIAN Mei(Research Institute of Horticulture,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002,Ningxia,China;Ningxia Hui Autonomous Region Agricultural Technology Extension General Station,Yinchuan 750001,Ningxia,China)
出处
《中国瓜菜》
北大核心
2025年第8期15-25,共11页
China Cucurbits And Vegetables
基金
宁夏回族自治区自然科学基金(2022AAC03442)
国家重点研发计划(2018YFD0201308)
宁夏重点研发计划(2018BBF02019,2022BBF02025)
宁夏农林科学院科技创新项目(NKYG-22-03,NGSB-2021-7-03)
国家西甜瓜产业技术体系专项(CARS-25)。
关键词
西瓜
嫁接砧木
转录组
代谢组
品质
Watermelon
Grafting rootstock
Transcriptome
Metabolome
Quality
