摘要
以29个酸枣品种(系)为试材,采用荧光毛细管电泳技术对其进行基因分型,研究了酸枣种质资源遗传多样性与种质亲缘关系,以期为辽宁酸枣核心种质构建提供参考依据。结果表明:12对SSR引物共检测到69个等位基因(Na),每对引物检测到2~8个Na。有效等位基因(Ne)在1.646~4.816,共有39.936个,占57.88%。Shannon信息指数(I)变幅介于0.552~1.668,平均值为1.258。观测杂合度(Ho)介于0.186~0.972,平均值为0.670。期望杂合度(He)介于0.372~0.789,平均值为0.663。不同引物揭示的多态信息含量(PIC)在0.372~0.835,平均为0.708。遗传相似系数在0~1,平均为0.28。聚类分析结果表明,在遗传距离为0.32时,可将29份酸枣种质分为四大类群。构建了酸枣指纹图谱及分子身份证,除2个品系外均具有唯一性。构建酸枣指纹图谱切实可行,可为酸枣品种身份识别、溯源管理及种质创新提供技术支撑和参考依据。
Taking 29 Z.jujuba varieties(lines)as the test materials,fluorescence capillary electrophoresis technology was used to genotype the genetic diversity and genetic relationships of Ziziphus jujuba germplasm resources was explored,in order to provide reference for the construction of core germplasm of Liaoning Ziziphus jujuba.The results showed that a total of 69 alleles(Na)were detected by 12 pairs of SSR primers,with 2-8 Na detected by each primer pair.The effective allele(Ne)ranged from 1.646 to 4.816,with a total of 39.936,accounting for 57.88%.The Shannon information index(I)ranged from 0.552 to 1.668,with an average of 1.258.The observed heterozygosity(Ho)ranged from 0.186 to 0.972,with an average of 0.670.The expected heterozygosity(He)ranged from 0.372 to 0.789,with an average of 0.663.The polymorphic information content(PIC)revealed by different primers ranged from 0.372 to 0.835,with an average of 0.708.The genetic similarity coefficient ranged from 0 to 1,with an average of 0.28.At the genetic distance of 0.36,the 29 Z.jujuba materials were divided into four categories by cluster analysis.Based on these results,we constructed a fingerprint and molecular ID card of Z.jujuba,which was unique to all but two strains.The construction of Z.jujuba fingerprint map was feasible and could provide technical support and reference basis for the identification,traceability management,and germplasm innovation of Z.jujuba varieties.
作者
李思琪
杨柳
付庆新
韩志立
张海旺
郭金龙
LI Siqi;YANG Liu;FU Qingxin;HAN Zhili;ZHANG Haiwang;GUO Jinlong(Institute of Agriculture and Forestry in Arid Areas of Liaoning Province,Chaoyang,Liaoning 122000;State Chaoyang Country Liujiazi Forest Farm,Chaoyang,Liaoning 122000;Zengcheng Agricultural Technology Promotion Center of Guangzhou City,Guangzhou,Guangdong 510000)
出处
《北方园艺》
北大核心
2025年第16期19-27,共9页
Northern Horticulture
基金
辽宁省农业科学院基本科研业务费协同创新专项资助项目(2025XTCX0402005)
辽宁省农业科学院旱地特色经济林学科建设计划资助项目(2025XKJS8560)
辽宁省林业和草原局2024年度依托国家林草局科技创新平台研发资助项目(LLC[2024]19)
中央财政林业科技推广示范资助项目(辽[2022]TG19号)。
关键词
酸枣
品种(系)
SSR荧光标记
遗传多样性
指纹图谱
分子身份证
Ziziphus jujuba
varieties(lines)
fluorescent labeled SSR markers
genetic diversity
fingerprints
molecular identity card
