摘要
本研究利用种子蛋白质聚丙烯酰胺凝胶电泳（ＰＡＧＥ）等多种电泳技术对供试西瓜杂交种及其亲本种子进行了鉴定，结果表明干种子蛋白质ＰＡＧＥ法在分离胶Ｔ＝１４％，Ｃ＝０．３５％，ｐＨ５．８；浓缩胶Ｔ＝４．０％，Ｃ＝０．３３％，ｐＨ等于５条件下可将供试西瓜杂交种与其亲本种子明显区分开，因此，这种方法可以作为该组合种子真实性鉴定的手段，作为纯度鉴定方法还需进一步研究。
Variety identification has always been the focal and difficult point of seed testing,especially for hybrid water melon seeds. Several kinds of electrophoresis techniques were used in this experiment to identify the hybrid water melon with its marternal and paternal seeds, and results showed that all these 3 varieties tested can be distingnished clearly by utilizing PAGE of dry seed proteins in the condition which T= 14%,C=0.35%, pH5.8 lower gel and T=4%,C=0.33%,pH5 upper gel. So the method can be used to identify the truthfulness of the varieties tested,but much work is being done to apply to genetic purity assay.
出处
《沈阳农业大学学报》
CAS
CSCD
1996年第1期92-93,共2页
Journal of Shenyang Agricultural University
关键词
PAGE法
西瓜
杂交种
蛋白质
PAGE method
Hybrid water melon seeds
Rrotein
