摘要
目的探讨三白草酮通过调控Hippo/YAP信号通路对非小细胞肺癌(NSCLC)细胞增殖和迁移的影响。方法使用不同浓度的三白草酮处理人NSCLC细胞系A549。采用CCK-8法检测细胞存活率,筛选药物浓度。将NSCLC细胞随机分为对照组和三白草酮低浓度组、三白草酮中浓度组、三白草酮高浓度组以及三白草酮高浓度+YAP激活剂(XMU-MP-1)组。使用CCK-8法、平板克隆实验、划痕实验、Transwell实验分别检测细胞存活率、细胞克隆形成情况、细胞迁移能力、细胞侵袭能力。采用蛋白质印迹检测迁移侵袭相关蛋白[E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、snail]及Hippo/YAP信号通路相关蛋白[Yes相关蛋白(YAP)、磷酸化Yes相关蛋白(p-YAP)、结缔组织生长因子(CTGF)、半胱氨酸丰富血管生成诱导因子61(CYR61)]的表达水平。结果与对照组比较,三白草酮低浓度组、三白草酮中浓度组、三白草酮高浓度组的细胞存活率、克隆细胞数量、细胞迁移和侵袭能力、N-cadherin和snail蛋白表达水平以及YAP、CTGF、CYR61蛋白表达水平降低或减少,E-cadherin及p-YAP蛋白表达水平升高,差异有统计学意义(P<0.05),其中三白草酮高浓度组的效果最好。与三白草酮高浓度组比较,三白草酮高浓度+YAP激活剂组的细胞存活率、克隆细胞数量、细胞迁移和侵袭能力、N-cadherin和snail蛋白表达水平以及YAP、CTGF、CYR61蛋白表达水平升高或增加,E-cadherin及p-YAP蛋白表达水平降低,差异有统计学意义(P<0.05)。结论三白草酮可能通过调控Hippo/YAP信号通路,降低NSCLC细胞存活率,抑制其增殖、迁移和侵袭能力。
Objective To explore the effect of saururus chinensis tannin on proliferation and migration of non-small cell lung cancer(NSCLC)cells by regulating Hippo/YAP signaling pathway.Methods Human NSCLC cell line A549 was treated with different concentrations of saururus chinensis tannin.Cell viability was detected by CCK-8 assay to screen appropriate drug concentrations.NSCLC cells were randomly divided into control group,low-concentration saururus chinensis tannin group,medium-concentration saururus chinensis tannin group,high-concentration saururus chinensis tannin group and high-concentration saururus chinensis tannin+YAP activator(XMU-MP-1)group.Cell viability,colony formation capacity,migration ability and invasion ability were evaluated using the CCK-8 assay,plate colony formation assay,wound healing assay and transwell assay,respectively.Expression levels of proteins associated with migration and invasion[E-cadherin,N-cadherin and snail]and proteins related to the Hippo/YAP signaling pathway[Yes-associated protein(YAP),phosphorylated Yes-associated protein(p-YAP),connective tissue growth factor(CTGF)and cysteine-rich angiogenic inducer 61(CYR61)]were detected by western blot analysis.Results Compared with the control group,the cell viability,the number of colony cells,migration and invasion abilities,as well as the expression levels of N-cadherin,snail,YAP,CTGF and CYR61 proteins in the low-concentration saururus chinensis tannin group,medium-concentration saururus chinensis tannin group and high-concentration saururus chinensis tannin group were significantly decreased or reduced,and the expression levels of E-cadherin and p-YAP proteins were significantly increased(P<0.05),with the most pronounced effects observed in the high-concentration saururus chinensis tannin group.Compared with the high-concentration saururus chinensis tannin group,the cell viability,the number of colony cells,migration and invasion abilities,as well as the expression levels of N-cadherin,snail,YAP,CTGF and CYR61 in the high-concentration saururus chinensis tannin+YAP activator group were significantly increased or enhanced,while the expression levels of E-cadherin and p-YAP were significantly decreased(P<0.05).Conclusion Saururus chinensis tannin may reduce the survival rate of NSCLC cells and inhibit their proliferation,migration and invasion abilities by regulating the Hippo/YAP signaling pathway.
作者
李玮
冯静波
郑运周
程琰
LI Wei;FENG Jingbo;ZHENG Yunzhou;CHENG Yan(Department of Laboratory Medicine,the 980th Hospital of the Joint Logistic Support Force of the People′s Liberation Army of China,Shijiazhuang,Hebei,050082;Department of Laboratory Medicine and Pathology,the 982nd Hospital of the Joint Logistic Support Force of the People′s Liberation Army of China,Tangshan,Hebei,063000;Basic Medical Research Laboratory,the 980th Hospital of the Joint Logistic Support Force of the People′s Liberation Army of China,Shijiazhuang,Hebei,050082)
出处
《实用临床医药杂志》
2025年第14期1-6,12,共7页
Journal of Clinical Medicine in Practice
基金
河北省2023年度医学科学研究课题(20231340)。
