摘要
目的研究温阳通络方对类风湿关节炎(rheumatoid arthritis,RA)成纤维样滑膜细胞(fibroblast-like synovial cells,FLS)上皮间质转化(epithelial-mesenchymal transition,EMT)的调控作用,探讨其作用机制。方法CCK-8实验筛选温阳通络方最佳含药血清浓度;将细胞分为正常关节成纤维样滑膜细胞(human fibroblast-like synovial cells,HFLS)组、人类风湿关节炎滑膜成纤维细胞(human rheumatoid arthritis synovial fibroblasts,MH7A)组、MH7A+温阳通络方含药血清(medicated serum,MS)组,Western Blot检测EMT相关蛋白E-钙黏蛋白(E-cadherin,E-cad)、纤维连接蛋白(fibronectin,FN)和波形蛋白(vimentin,VM)的表达;CCK-8实验检测各组细胞活性;Transwell实验检测各组细胞迁移和侵袭能力。结果96 h时15%温阳通络方含药血清干预后MH7A细胞活性最接近正常细胞的一半,即半抑制浓度(half maximal inhibitory concentration,IC50);MH7A细胞中E-cad的表达水平降低(P<0.05)、FN和VM的表达水平升高(P<0.05),细胞的活性、迁移和侵袭能力增强(P<0.05);温阳通络方含药血清干预MH7A细胞后,细胞中E-cad的表达水平升高(P<0.05)、FN和VM的表达水平降低(P<0.05),细胞的活性、迁移和侵袭能力减弱(P<0.05)。结论温阳通络方可能通过抑制MH7A的EMT现象,抑制滑膜细胞的活性、迁移和侵袭,从而发挥对RA的治疗作用。
Objective To study the regulatory effect of Wenyang Tongluo Prescription(温阳通络方)on the epithelial-mesenchymal transition(EMT)of fibroblast-like synovial(FLS)cells in rheumatoid arthritis(RA)and to explore its mechanism.Methods CCK-8 test was used to screen the optimal serum concentration of Wenyang Tongluo Prescription.The cells were divided into human fibroblast-like synovial cells(HFLS)group,human rheumatoid arthritis synovial fibroblasts(MH7A)group,MH7A+Wenyang Tongluo Prescription medicated serum(MS)group.The expressions of EMT-related proteins E-cadherin(E-cad),Fibronectin(FN)and Vimentin(VM)were detected by Western Blot.Cell activity was detected by CCK-8 assay.The ability of cell migration and invasion was detected by Transwell assay.Results After intervention of 15%serum containing Wenyang Tongluo Prescription at 96 h,the activity of MH7A cells was mostly close to half of that of normal cells,that is,half maximal inhibitory concentration(IC 50).The expression level of E-cad in MH7A cells was decreased(P<0.05),the expression levels of FN and VM were increased(P<0.05),and the cell activity,migration and invasion ability increased(P<0.05).After intervention of MH7A cells with serum containing Wenyang Tongluo Prescription,the expression level of E-cad in MH7A cells was increased(P<0.05),the expression levels of FN and VM decreased(P<0.05),and the activity,migration and invasion ability of cells were decreased(P<0.05).Conclusion Wenyang Tongluo Prescription may inhibit the activity,migration and invasion of synovial cells by inhibiting the EMT phenomenon of MH7A,so as to play a therapeutic role in RA.
作者
田亚丹
李冬艳
罗定霞
宋佳欣
吴晶金
TIAN Yadan;LI Dongyan;LUO Dingxia;SONG Jiaxin;WU Jingjin(The First Clinical College of Yunnan University of Traditional Chinese Medicine,Kunming 650011,Yunnan,China;Yunnan Provincial Hospital of Traditional Chinese Medicine,Kunming 650021,Yunnan,China)
出处
《辽宁中医杂志》
北大核心
2025年第8期167-170,I0001,I0002,共6页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(82360924)
云南省中医联合专项重点项目(202101AZ070001-161)
兴滇英才“青年人才”项目
云南省高层次中医痹病学后备人才项目。
关键词
温阳通络方
类风湿关节炎
成纤维样滑膜细胞
上皮间质转化
Wenyang Tongluo Prescription(温阳通络方)
rheumatoid arthritis
fibroblast-like synovial cells
epithelial mesenchymal transformation
