摘要
以本项目组前期从玉米蛋白水解物中分离纯化得到的玉米蛋白单肽KSCAPLAS为研究对象,通过构建乙醇诱导LO2细胞损伤模型,探究玉米肽KSCAPLAS的拮抗乙醇性损伤作用。结果表明,KSCAPLAS能够显著降低乙醇诱导损伤的LO2细胞内活性氧(ROS)的含量(P<0.05),并显著提高超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GR)活性和还原型谷胱甘肽(GSH)水平(P<0.05),对乙醇性损伤的LO2细胞具有拮抗作用。此外,KSCAPLAS通过上调线粒体呼吸链复合物Ⅰ的含量进而上调细胞内ATP含量。与对照组相比,在肽质量浓度为100μg/mL条件下,细胞内线粒体呼吸链复合物Ⅰ和ATP的质量分数分别提高了63.11%和31.65%。玉米肽KSCAPLAS通过促进乙醇性损伤细胞代谢提供能量,进而缓解乙醇诱导LO2细胞的损伤。
KSCAPLAS,a single peptide isolated and purified from corn protein hydrolysate,was investigated to explore the antagonistic effect of KSCAPLAS on ethanol-induced damage of LO2 cells by constructing an ethanol-induced cellular damage model.The results showed that KSCAPLAS could significantly reduce the content of reactive oxygen species(ROS)in ethanol-induced LO2 cells(P<0.05),and significantly increase the activites of superoxide dismutase(SOD)and glutathione reductase(GR),and glutathione(GSH)levels(P<0.05),as well as had antagonistic effects on ethanol-induced LO2 cell damage.In addition,KSCAPLAS was found to upregulate intracellular ATP content by increasing the level of mitochondrial respiratory chain complex I.Compared with the control group,at a peptide concentration of 100μg/mL,the mass fractions of mitochondrial respiratory chain complexⅠand ATP were increased by 63.11%and 31.65%,respectively.Maize peptide KSCAPLAS provides energy by promoting the metabolism of ethanol-induced LO2 cells,thereby alleviating ethanol-induced LO2 cell damage.
作者
崔宁
李冠龙
刘晓兰
郑喜群
Cui Ning;Li Guanlong;Liu Xiaolan;Zheng Xiqun(College of Food and Bioengineering,Qiqihar University,Qiqihaer161006;Heilongjiang Provincial Key Laboratory of Corn Deep Processing Theory and Technology,Qiqihaer161006;College of Food,Heilongjiang Bayi Agricultural University,Daqing163319)
出处
《中国粮油学报》
北大核心
2025年第6期77-84,共8页
Journal of the Chinese Cereals and Oils Association
基金
中央引导地方科技发展专项(ZY23QY06)
黑龙江省省属高等学校基本科研业务费科研项目(145409456)。
关键词
玉米肽
抗氧化
LO2细胞
能量代谢
corn peptide
anti-oxidation
LO2 cells
energy metabolism
