摘要
以脱脂玉米胚芽粕为原料,初步探索了玉米胚芽粕的单酶水解、双酶分步水解条件,并对酶解产物的抗氧化活性进行了初步研究。确定了玉米胚芽粕水解蛋白的提取工艺参数。采用纤维素酶和碱性蛋白酶对玉米胚芽粕进行两步酶法处理,通过单因素试验和正交试验设计,对提取工艺参数进行优化。结果表明,纤维素酶酶解的最佳工艺为:酶解pH 4.8、酶解时间2.5 h、酶解温度50℃、加酶量0.5%。碱性蛋白酶酶解的最佳工艺为:酶解pH 9.5、酶解时间4.0 h、酶解温度40℃、加酶量5%。在该条件下,玉米胚芽粕水解蛋白的提取率可达到68.48%。初步研究了酶解产物抗氧化活性,包括清除DPPH、羟自由基能力和还原力。结果表明,酶解产物具有较强的还原力,且对DPPH和羟基自由的IC50分别为5.223μg/mL和32.030μg/mL,说明该产物具有较好的抗氧化活性。
Using defatted corn germ as raw material, the condition of the single enzyme hydrolysis and composite double enzyme hydrolysis of corn germ was explored, and the antioxidant activity of enzymatic products preliminarily was studied. The technology parameters were determined. Cellulose and alkaline protease were used in the extraction of corn germ protein. Single factor experiment was designed to get a technological parameter. The optimal processing conditions for cellulose were reaction pH 4.8, reaction time 2.5 h, reaction temperature 50 ℃, and the amount of enzyme 0.5%;And the optimal processing conditions for alkaline protease are reaction pH 9.5, reaction time 4.0 h, reaction temperature 40 ℃, and the amount of enzyme 5%. In the condition, the extraction percentage was 68.48%. The antioxidant activity of the enzymatic products was studied, including the ability of removing DPPH, hydroxyl radicals and reducing preliminarily. The result showed that strong reducing power was observed. In DPPH and hydroxyl free radical scavenging assays, the IC50 was 5.223 μg/mL and 32.030 μg/mL, respectively. The results supported strong antioxidant activity in vitro for enzymatic products.
作者
马雪
关海宁
郭丽
李文杰
李杨
侯艳丽
MA Xue;GUAN Haining;GUO Li;LI Wenjie;LI Yang;HOU Yanli(College of Food and Pharmaceutical Engineering, Suihua University,Suihua 152061)
出处
《食品工业》
CAS
北大核心
2019年第7期85-90,共6页
The Food Industry
基金
2017年度黑龙江省省属高等学校基本科研业务费科研项目(2017-KYYWF-0724)
关键词
玉米胚芽粕
水解蛋白
酶解
抗氧化活性
corn germ meal
protein
enzymatic hydrolysis
antioxidant activity
