摘要
杨树快速繁殖与遗传转化体系是解析木本植物基因功能与杨木遗传改良的关键技术,其效率受限于愈伤组织再生与分化能力。本研究在以杂交杨为底盘转化白藜芦醇合成酶基因(PcRS)过表达载体时,发现受伤叶脉处形成愈伤组织的量比其他载体更多,分化的时间显著提前,同时PcRS过表达杨树的叶脉中积累的白藜芦醇含量高达8.509μg·g^(-1)。外源施加不同浓度的白藜芦醇可不同程度提升杨树叶片诱导愈伤组织的生物量,在最适浓度60μmol·L^(-1)白藜芦醇处理下,愈伤组织的生物量增长最高可达对照的210.7%。联合转录组和激素组分析发现施加白藜芦醇可激活细胞分裂素生物合成通路,导致3种活性细胞分裂素组分含量上升;同时部分抑制生长素的合成通路,但对生长素积累量影响较小。这些结果表明白藜芦醇影响杨树愈伤组织诱导过程中的激素平衡,提高了植物体内细胞分裂素/生长素的比例,有效促进愈伤组织的生长。本研究揭示了白藜芦醇在调控杨树愈伤组织生长的作用,为优化杨树快速繁殖与遗传转化体系提供了新的技术方案。
The rapid propagation and genetic transformation system of Populus constitutes a pivotal technology for deciphering gene functions in woody plants and advancing poplar genetic improvement,with its efficiency constrained by the callus regeneration and differentiation capacity.In this study,we observed enhanced callus formation at wounded leaf veins accompanied by significantly accelerated differentiation timing when aspen was employed as the host plant for transforming a construct containing a resveratrol synthetase gene(PcRS)overexpression cassette.Specifically,the resveratrol content accumulated in the leaf veins of PcRS-overexpressing poplar reached 8.509μg·g^(-1).Exogenous application of varying concentrations of resveratrol differentially enhanced callus biomass induced from poplar leaves,with optimal efficacy at 60μmol·L^(-1)resveratrol treatment where callus biomass increased up to 210.7%of the control group.Integrated transcriptomic and hormone metabolomic analysis revealed that resveratrol application activated the cytokinin biosynthesis pathway,leading to increased levels of three active cytokinin components,while partially suppressing auxin biosynthesis pathways without substantially affecting auxin accumulation.These findings demonstrate that resveratrol modulates the hormonal homeostasis during poplar callus induction by elevating the cytokinin/auxin ratio,thereby effectively promoting callus proliferation and differentiation.This study reveals the role of resveratrol in regulating poplar callus growth,and provides a novel technical approach for optimizing the rapid propagation and genetic transformation system of poplar.
作者
林华娜
范宇欣
卫国琴
李筱
陈鸿鹏
赵运军
LIN Huana;FAN Yuxin;WEI Guoqin;LI Xiao;CHEN Hongpeng;ZHAO Yunjun(College of Chinese Materia Medica,Guangdong Pharmaceutical University,Guangzhou 510006,China;Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China;College of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou 311300,China)
出处
《植物生理学报》
北大核心
2025年第6期729-738,共10页
Plant Physiology Journal
基金
科技创新2030-重大项目(2023ZD04069)
中国科学院基础与交叉前沿科研B类先导专项(XDB0630203)。
