摘要
通过对3种抗生素的筛选,确立最适抑制农杆菌过度生长、促进微型月季愈伤组织生长与分化的抗生素及其浓度,从而建立稳定而有效的微型月季遗传转化体系。结果表明:微型月季叶片培养于150 mg/L头孢噻肟钠培养基可抑制农杆菌生长且利于材料生长分化。侵染最佳条件为:侵染菌液OD_(600)为0.5—0.6,侵染5—10 min,共培养2 d。‘荷仙姑’‘彩虹’和‘笑脸’微型月季阳性愈伤的PCR阳性率分别为26.4%,18.4%和31.2%。该研究结果为微型月季农杆菌遗传转化的抗生素选择和培育条件的优化提供一定参考。
By using three antibiotics(cefotaxime sodium,carbenicillin and ampicillin),the most suitable antibiotics and their concentrations for inhibiting excessive growth of Agrobacterium and promoting the growth and differentiation of miniature rose leaf callus were determined,thus to establish a stable and effective genetic transformation system for miniature roses.The results indicated that the transforming leaves cultured in 150 mg/L cefotaxime sodium medium could inhibit the growth of Agrobacterium and promote callus growth and differentiation.The optimal conditions for infection were as follows:an OD 600 value of 0.5—0.6 in the infection solution,infection duration of 5—10 min,and co-cultivation for 2 days.The PCR positive transformants rates of‘Hexiangu’‘Rainbow’and‘Smiling Face’miniature rose positive callus were 26.4%,18.4%,and 31.2%,respectively.The results could provide some reference for the selection of antibiotics and optimization of cultivation conditions in the genetic transformation of Agrobacterium.
作者
李燕
黄婧
张启香
张敏
Li Yan;Huang Jing;Zhang Qixiang;Zhang Min(Zhejiang A&F University,Hangzhou 311300,China;Jiangsu Academy of Forestry,Nanjing 211153,China)
出处
《江苏林业科技》
2025年第2期1-9,共9页
Journal of Jiangsu Forestry Science & Technology
基金
江苏省林业科学研究院自主科研项目“无(稀)皮刺月季新品种选育”(ZZKY202302)
江苏省省级作物种质圃(月季)保种项目(2024-SJ-021)
江苏省科技资源(农业种质)统筹服务平台月季种质资源圃。
关键词
微型月季
遗传转化
抗生素
培养
农杆菌
Miniature rose
Genetic transformation
Antibiotic
Tissue culture
Agrobacterium
