摘要
目的:观察三七总皂苷(PNS)对心肌缺血再灌注(IR)损伤的作用,探讨其机制是否与调控心肌细胞焦亡有关。方法:采用随机数字表法将18只成年雄性SD大鼠分为假手术组、IR组和PNS组,各6只。采用结扎大鼠冠状动脉左前降支(LAD)制备模型,缺血30 min,再灌注2 h;假手术组和IR组大鼠腹腔注射生理盐水(10 mL/kg),PNS组于造模前30 min腹腔注射PNS(50 mg/kg)。采用苏木精-伊红(HE)染色观察心肌损伤程度;免疫组化法检测半胱氨酸天冬氨酸特异性蛋白酶-1(Caspase-1)表达;蛋白免疫印记法(Western Blot)检测心肌组织Caspase-1、消化道皮肤素D(GSDMD)蛋白含量的表达。结果:与假手术组比较,IR组心肌损伤程度加重,Caspase-1、GSDMD蛋白表达增加(P<0.05);与IR组比较,PNS组心肌损伤程度减轻,Caspase-1、GSDMD蛋白表达降低(P<0.05)。结论:三七总皂苷可减轻大鼠心肌IR损伤,其作用机制可能与抑制心肌细胞焦亡有关。
Objective:To observe the effect of Panax notoginseng saponins(PNS)on myocardial ischemia-reperfusion(IR)injury and explore its mechanism related to the regulation of cardiomyocyte pyroptosis.Methods:Eighteen adult male SD rats were divided into sham group,IR group and PNS group by random number table method,with 6 rats in each group.The model was prepared by ligating the left anterior descending coronary artery(LAD)of rats,with ischemia for 30 minutes and reperfusion for 2 hours.The rats in the sham group and the IR group were intraperitoneally injected with normal saline(10 mL/kg),while those in the PNS group were intraperitoneally injected with PNS(50 mg/kg)30 minutes before modeling.The degree of myocardial injury was observed by hematoxylin-eosin(HE)staining.The expression of cysteine aspartate specific protease-1(Caspase-1)was detected by immunohistochemistry.The expressions of Caspase-1 and gasdermin D(GSDMD)protein contents in myocardial tissue were detected by Western Blot.Results:Compared with the sham group,the degree of myocardial injury in the IR group was aggravated,and the protein expressions of Caspase-1 and GSDMD increased(P<0.05).Compared with the IR group,the degree of myocardial injury in the PNS group was alleviated,and the protein expressions of Caspase-1 and GSDMD decreased(P<0.05).Conclusion:Panax notoginseng saponins could alleviate myocardial IR injury in rats,and its mechanism might relate to the inhibition of cardiomyocyte pyroptosis.
作者
赵位昆
徐彤彤
吕祥威
覃泱
路凤霞
李于庭
黄宇莉
莫琪
徐华欣
吕菲
ZHAO Weikun;XU Tongtong;LYU Xiangwei;QIN Yang;LU Fengxia;LI Yuting;HUANG Yuli;MO Qi;XU Huaxin;LYU Fei(The First Affiliated Hospital of Guilin Medical University,Guilin 541001,Guangxi,China;Guilin Medical University,Guilin 541199,Guangxi,China)
出处
《中西医结合心脑血管病杂志》
2025年第14期2128-2132,共5页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
基金
国家自然科学基金项目(No.82260864)
广西自然科学基金项目(No.2018GXNSFAA050110,2025GXNSFAA069850)
广西壮族自治区中医药管理局自筹经费科研课题项目(No.GXZYC20240401)
国家级大学生创新创业训练计划项目(No.202110601004)
校级大学生创新创业训练计划项目(No.202110601165)。
