摘要
目的:研究不同年龄的自发性高血压大鼠(SHR)和WistarKyoto大鼠(WKY)主动脉平滑肌中丝裂原活化蛋白激酶(MAPK)及其磷酸酶(MKP-1)的表达及其与高血压的关系。方法:用tail-cuff测量大鼠尾动脉血压;分别用Westernblotting法和RT-PCR法半定量测定血管平滑肌中磷酸化细胞外信号调节激酶(p-ERK)和MKP-1的蛋白表达以及MKP-1mRNA的含量。结果:(1)SHR的血压自8周龄起明显高于WKY(P<0.01),且随年龄增长而升高(P<0.05)至14周以后趋于稳定;(2)SHR主动脉平滑肌中的p-ERK表达明显高于同年龄的WKY(P<0.01),随年龄增长而递增(P<0.05),与血压呈正相关;(3)SHR主动脉平滑肌中MKP-1蛋白明显高于同龄WKY,而mRNA的表达在5周龄时明显高于WKY,之后均随年龄的增长而递减(P<0.05),与血压和ERK呈负相关,而WKY下降不明显。结论:MKP-1在高血压的发生和发展过程中起重要作用,其表达逐渐下降可能是导致ERK激活增加,从而导致血管平滑肌细胞增殖、血压升高的重要原因。
AIM： To investigate the expression of mitogen - activated protein kinase and mitogen - activated protein kinase phosphatase- 1 in thoracic aorta smooth muscles of spontaneously hypertensive rats （SHR） and Wistar Kyoto rats （WKY） with different ages and the relationship between those and hypertension. METHODS： The caudal arterial pressure was measured by tail- cuff. Protein expression of p- ERK was detected by Western blotting, and MKP- 1 mRNA in thoracic aorta smooth muscle was examined by RT- PCR. RESULTS： （ 1 ） The blood pressure of SHR was obviously higher than that of age - matched WKY （ P 〈 0.01 ）, elevated with age （ P 〈 0.05） and became stable from 14- week - old. （2） The expression of p- ERK and MKP- 1 in SHR was higher than that in WKY in 5 - week - old rats, and the expression of p - ERK increased with age, while the expres- sion of MKP - 1 decreased with age （ P 〈 0.05）. CONCLUSION： MKP - 1 may play an important role in the development of hypertension in SHR. The decrease in the expression of MKP- 1 that resulted in the activation of MAPK may induct vascular smooth muscle proliferation and hypertrophy.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第3期468-471,共4页
Chinese Journal of Pathophysiology
基金
浙江省科技厅资助项目(No.021107057)
浙江省自然科学基金资助项目(M303874)
