摘要
[目的]建立一种离子色谱同时测定婴幼儿配方乳粉中6种母乳寡聚糖(HMOs)的分析方法。[方法]样品经果聚糖酶酶解,以水-0.5 moL/L氢氧化钠-0.3 mol/L乙酸钠为流动相梯度洗脱,Thermo Fisher CarboPacTM PA1 Analytical Column分离柱分离,采用离子色谱—脉冲安培法测定。[结果]乳粉中2'-岩藻糖基乳糖(2'-FL)、3-岩藻糖基乳糖(3-FL)、乳糖-N-四糖(LNT)在1~100 mg/L的线性范围内线性良好,乳糖-N-新四糖(LNnT)、3'-唾液酸乳糖(3'-SL)、6'-唾液酸乳糖(6'-SL)在0.5~50.0 mg/L的线性范围内线性良好,相关系数均>0.999。2'-FL、LNnT、3-FL、LNT的检出限为10 mg/100 g,定量限为30 mg/100 g;3'-SL、6'-SL的检出限为5 mg/100 g,定量限为15 mg/100 g。6种HMOs在低、中、高浓度下的平均回收率为98.0%~101.0%,相对标准偏差为0.3%~2.4%。在实际样品检测中,当6种HMOs含量为0.1~10.0 g/kg时,RSD为0.5%~9.4%;当6种HMOs含量为10~100 g/kg时,RSD为1.4%~4.0%。[结论]该方法可满足日常检测需求,适用于多种含HMOs婴配乳粉中6种母乳寡聚糖的测定。
[Objective]To establish an ion chromatography method for the simultaneous determination of six human milk oligosaccharides(HMOs)in infant formula powder.[Methods]The samples were enzymatically hydrolyzed using fructanase.Gradient elution was performed with a mobile phase consisting of water,0.5 mol/L sodium hydroxide,and 0.3 mol/L sodium acetate.Separation was carried out on a Thermo Fisher CarboPac™PA1 Analytical Column,and detection was performed using ion chromatography with pulsed amperometric detection.[Results]In milk powder,2'-fucosyllactose(2'-FL),3-fucosyllactose(3-FL),and lacto-N-tetraose(LNT)showed good linearity within the range of 1~100 mg/L,while lacto-N-neotetraose(LNnT),3'-sialyllactose(3'-SL),and 6'-sialyllactose(6'-SL)exhibited good linearity in the range of 0.5~50.0 mg/L,with correlation coefficients all greater than 0.999.The limit of detection(LOD)for 2'-FL,LNnT,3-FL,and LNT was 10 mg/100 g,and the limit of quantification(LOQ)was 30 mg/100 g.The LOD for 3'-SL and 6'-SL was 5 mg/100 g,and the LOQ was 15 mg/100 g.The average recoveries of the six HMOs at low,medium,and high concentrations ranged from 98.0%to 101.0%,with relative standard deviations(RSDs)between 0.3%and 2.4%.In actual sample analysis,when the content of the six HMOs ranged from 0.1 to 10.0 g/kg,the RSDs were 0.5%to 9.4%.When the content ranged from 10 to 100 g/kg,the RSDs were 1.4%to 4.0%.[Conclusion]The proposed method meets the requirements for routine analysis and is suitable for the determination of six HMOs in various infant formula powders containing HMOs.
作者
张嘉杞
李云萱
王璐璐
张水锋
潘项捷
施元旭
阙利明
ZHANG Jiaqi;LI Yunxuan;WANG Lulu;ZHANG Shuifeng;PAN Xiangjie;SHI Yuanxu;QUE Liming(Zhejiang Fangyuan Test Group Co.,Ltd.,Hangzhou,Zhejiang 310018,China)
出处
《食品与机械》
北大核心
2025年第5期64-70,共7页
Food and Machinery
基金
浙江省市场监督管理局科技计划(编号:ZC2023046)。
关键词
离子色谱法
酶解法
母乳寡聚糖
婴幼儿配方乳粉
ion chromatography
enzymolysis
human milk oligosaccharides
infant formula milk powder
