摘要
用离子色谱法建立一种简单、快速、准确、灵敏的半乳糖醛酸和葡萄糖醛酸的测定方法.多糖水解液经过简单的中和、稀释、过滤后直接进样分析.半乳糖醛酸和葡萄糖醛酸具有较高的分离度(R=4.2);两者在20～200 mg/L质量浓度范围内都具有良好的线性,其相关系数均为r=0.999 0;重复性和回收率都满足了仪器分析要求;两种化合物的检测限分别为100 ng和50 ng.
A simple, fast, accurate and highly sensitive method for the determination of galacturonic acid and glucuronic acid in the hydrolyzed polysaccharide solution by Ion Chromatography-PAD was established. After neutralization, dilution, and filtration, the hydrolyzed polysaccharide solution was injected directly. Galacturonic acid and glucuronic acid exhibited good resolution （R=4.2）. Two linear regression equations of y=27 469x＋136 911（r =0. 999 0）and y=66 379x＋347 163（ r=0. 999 0）were obtained for galacturonic and glucuronic acids, respectively. The linear ranges of the two compounds were 20 ～ 200 mg/L. The good accuracy and recovery rate met the demands of the instrument analysis. The limit of detection was 0. 1μg and 0. 05μg, respectively.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2005年第4期86-88,共3页
Journal of Food Science and Biotechnology
关键词
离子色谱
半乳糖醛酸
葡萄糖醛酸
脉冲安培检测器
ion chromatography（IC）
galacturonic acid
glucuronic acid
pulse ampere detector（PAD）
