摘要
[目的]分离纯化并鉴定引起羊肚菌Morchella spp.腐病的致病菌,筛选出对其具有较好防治作用的复配农药。[方法]采用组织分离法分离纯化并鉴定羊肚菌腐病的致病菌,采用菌丝生长速率法测定24种农药单剂及4种复配剂在不同配比下对其的生物活性,筛选出联合作用最好的复配组合及比例,并在此基础上进行大田试验。[结果]从羊肚菌患病组织中分离纯化出致病菌木霉菌,通过药剂筛选发现90%多菌灵WG与0.3%四霉素AS以6∶1的比例复配时对其表现出增效作用,增效系数SR为1.83,大田试验防效为72.73%。[结论]多菌灵和四霉素联合复配使用为生产上防控羊肚菌腐病提供了绿色科学的有效策略。
[Aims]The aim of this study is to isolate,purify and identify the pathogen causing rot disease of Morchella spp.,and screen fungicidal compositions with good control effect on it.[Methods]The pathogenic fungus of Morchella spp.rot was isolated,purified and identified by tissue isolation method.The mycelial growth rate method was used to determine the biological activities of 24 pesticides and 4 pesticide combinations under different ratios against the pathogen,to screen out the combinations and ratios with the best combined effects,and to carry out field tests on this basis.[Results]The pathogenic fungus Trichoderma spp.was isolated and purified from the diseased tissues of Morchella spp.,and combinition of carbendazim 90%WG and tetramycin 0.3%AS in the ratio of 6:1 exhibited synergistic effect,with the synergistic coefficient(SR)of 1.83 and the field control efficacy of 72.73%.[Conclusions]The joint use of carbendazim and tetramycin provides a green,scientific and effective strategy for the prevention and control of Morchella spp.rot in production.
作者
耿朋
曹立倩
张邦喜
蔡琴
胡淑慧
艾燕琴
吴文能
GENG Peng;CAO Liqian;ZHANG Bangxi;CAI Qin;HU Shuhui;AI Yanqin;WU Wenneng(School of Food Science and Engineering,Guiyang University,Guiyang 550005,China;Institute of Soil and Fertilizer Research,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;Weining County Qian-he Agricultural Products Co.,Ltd.,Bijie 553100,Guizhou,China)
出处
《农药》
北大核心
2025年第5期380-385,390,共7页
Agrochemicals
基金
贵州省科技支撑项目(黔科合支撑[2022]一般132和黔科合支撑[2023]一般082)
贵州省科技支撑项目(黔科合平台人才-YQK[2023]024)
贵州省科技支撑项目(黔科合平台人才-CARS-20)。
关键词
羊肚菌
木霉菌
分离鉴定
活性测试
农药复配
Morchella spp.
Trichoderma spp.
separation and identification
activity test
pesticide combination
