摘要
采用硅胶柱、凝胶Sephadex LH20柱、p-HPLC等多种色谱法对圆叶乌桕(Triadica rotundifolia)乙醇提取物的乙酸乙酯部位进行分离纯化,共从中获得23个化合物。运用核磁等波谱法并结合文献数据对比分析对分离得到的单体化合物进行结构鉴定,分别确定为飞龙掌血内酯酮(1)、飞龙掌血内酯(2)、莨菪亭(3)、6,7-二甲氧基香豆素(4)、6,7,8-三甲氧基香豆素(5)、8-羟基-5,6,7-三甲氧基香豆素(6)、5,6,7,8-四甲基香豆素(7)、lup-20(29)-ene-3α,23-diol(8)、算盘子酮(9)、3β-O-trans-caffeoylbetulinic acid(10)、oleana-1,18-dien-3-one(11)、morolic acid acetate(12)、β-谷甾醇(13)、胡萝卜苷(14)、6-hydroxystigmast-4-en-3-one(15)、oleodaphnone(16)、5′-甲氧基川陈皮素(17)、trigonochinene E(18)、没食子酸乙酯(19)、瑞香毒素B(20)、(+)-3-hydroxy-1,5-diphenyl-lpentanone(21)、5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one(22)和吲哚-3-甲醛(23)。已知化合物1~23均为首次从圆叶乌桕中分离得到。对分离得到的所有单体化合物进行初步细胞毒活性筛选,结果显示化合物8和18对非小细胞肺癌细胞H1975具有中等强度的细胞毒活性,其IC50值分别为(13.97±0.40)和(26.61±1.81)μmol/L。
In this study,a total of 23 compounds were obtained from the ethyl acetate part of Triadica rotundifolia by various chromatographic methods such as silica gel,Sephadex LH20,and p-HPLC column chromatography.Their structures were identified by NMR spectroscopy combined with comparative analysis with literature data as known compounds:toddanone(1),toddalolactone(2),scopoletin(3),6,7-dimethoxycoumarin(4),6,7,8-trimethoxycoumarin(5),8-hydroxy-5,6,7-trimethoxycoumarin(6),5,6,7,8-tetramethoxycoumarin(7),lup-20(29)-ene-3α,23-diol(8),glochidone(9),3β-O-trans-caffeoylbetulinic acid(10),oleana-1,18-dien-3-one(11),morolic acid acetate(12),β-sitosterol(13),daucosterol(14),6-hydroxystigmast-4-en-3-one(15),oleodaphnone(16),5′-methoxynobiletin(17),trigonochinene E(18),ethyl gallate(19),daphnelantoxin B(20),(+)-3-hydroxy-1,5-diphenyl-l-pentanone(21),5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one(22),and indole-3-carbaldehyde(23).Compounds 1-23 were isolated from T.rotundifolia for the first time.In addition,activity screening revealed that compounds 8 and 18 showed moderate cytotoxicity to H1975 cells,with IC50 values of(13.97±0.40)μmol/L and(26.61±1.81)μmol/L,respectively.
作者
陈佳倩
崔清雅
吴雷名
陈贤安
郑冰莹
黄冬
唐贵华
CHEN Jiaqian;CUI Qingya;WU Leiming;CHEN Xian’an;ZHENG Bingying;HUANG Dong;TANG Guihua(School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006,China)
基金
广州市科技计划项目(2024B03J1322)
广东省科技计划项目(2023A1111120025)。
