摘要
对来源于南海表层海水的杂色曲霉真菌Aspergillus versicolor NJF3的大米发酵物进行了次级代谢产物的研究。该菌株的大米固体发酵物用φ=95%乙醇提取后经过萃取和色谱分离纯化,共获得15个单体成分,其结构经过波谱数据分析分别鉴定为:meleagrin(1)、fumigaclavine C(2)、cyclo(L-Trp-L-Phe)(3)、phenazine-1-carboxylic acid(4)、phenazine-1-carboxamide(5)、N-(2-hydroxypropanoyl)-2-aminobenzoic acid amide(6)、orlandin(7)、monomethylsulochrin(8)、sydowinin A(9)、diorcinol-3-O-α-D-ribofuranoside(10)、cordyol C-3-O-α-D-ribofuranoside(11)、3,3'-dihydroxy-5,5'-dimethyldiphenyl ether(12)、3,5-dihydroxytoluene(13)、3,7-dihydroxy-1,9-dimethyldibenzofuran(14)和p-hydroxyphenylacetic acid methyl ester(15)。所有化合物均为已知结构,其中二苯醚类糖苷化合物10和11为首次从杂色曲霉的次级代谢产物中分离得到。另外,活性筛选结果显示化合物1对非小细胞肺癌细胞具有中等细胞毒性。
The secondary metabolites of the rice fermentation of Aspergillus versicolor NJF3,a fungus derived from the surface seawater of the South China Sea,were investigated in this study.The rice solid fermentation product of this strain was extracted with 95%ethanol and subjected to ethyl acetate extraction,followed by repeated chromatographic separation and purification.As a result,a total of 15 compounds were obtained,and their structures were identified by spectral data analysis as meleagrin(1),fumigaclavine C(2),cyclo(L-Trp-L-Phe)(3),phenazine-1-carboxylic acid(4),phenazine-1-carboxamide(5),N-(2-hydroxypropanoyl)-2-aminobenzoic acid amide(6),orlandin(7),monomethylsulochrin(8),sydowinin A(9),diorcinol-3-O-α-D-ribofuranoside(10),cordyol C-3-O-α-D-ribofuranoside(11),3,3'-dihydroxy-5,5'-dimethyldiphenyl ether(12),3,5-dihydroxytoluene(13),3,7-dihydroxy-1,9-dimethyldibenzofuran(14),and p-hydroxyphenylacetic acid methyl ester(15),respectively.All compounds were known structures,among them diphenyl ether glycosides 10 and 11 were isolated from secondary metabolites of A.variegatus for the first time.In addition,activity screening showed that compound 1 moderate cytotoxicity to NSCLC cells.
作者
卢清仁
吴雷名
翁汉桩
唐贵华
LU Qingren;WU Leiming;WENG Hanzhuang;TANG Guihua(School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006,China)
基金
南方海洋科学与工程广东省实验室(珠海)自主科研项目(SML2021SP301)。
关键词
海洋真菌
次级代谢产物
曲霉
细胞毒
marine fungi
secondary metabolites
Aspergillus
cytotoxicity
