摘要
目的探讨陈皮酵素(Pericarpium citri reticulatae fermentation liquor,PF)对小鼠酒精性肝损伤和肠道的调节作用。方法将40只C57BL/6雄性小鼠随机分为正常组、模型组、阳性药物组[100mg/(kg·d)水飞蓟宾(silibinin,Sly)]和陈皮酵素组[500 mg/(kg·d)PF],每组10只,持续给药干预2周。通过分子生物学检测生物化学和病理学指标;16S rDNA高通量测序技术检测分析粪便微生物群。结果组织切片结果显示陈皮酵素可以显著改善酒精性肝损伤小鼠的肝脏和回肠组织损伤,减少酒精诱导的肝脏脂质积累。此外,陈皮酵素显著降低肝脏组织促炎因子[白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-6(interleukin-6,IL-6)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)]的水平,缓解酒精引起的氧化应激。同时,陈皮酵素可修复酒精引起的肠道菌群紊乱,维持肠道菌群数量,修复肠道黏膜屏障损伤。结论陈皮酵素可改善酒精性肝损伤,其机制与抗氧化、抗炎和调节肠道菌群紊乱有关,值得推广应用与开发。
Objective To investigate the regulatory effects of Pericarpium citri reticulatae fermentation liquor(PF)on alcoholic liver disease and intestinal tract in mice.Methods Forty male C57BL/6 mice were randomly divided into 4 groups,including a normal group,model group,positive drug group[100 mg/(kg·d)silibinin(Sly)],and PF group[500 mg/(kg·d)PF],with 10 mice in each group,continuous medication intervention for 2 weeks.Biochemical and pathological indicators were assessed using molecular biology techniques.Fecal microbiota was analyzed using 16S rDNA high-throughput technology.Results Histological analysis revealed that PF significantly ameliorated alcohol-induced liver and ileum tissue damage in mice,while reducing alcohol-induced liver lipid accumulation.Furthermore,PF effectively reduced pro-inflammatory factors levels(IL-1β,IL-6,TNF-α)in liver tissue and alleviated alcohol-induced oxidative stress.Additionally,PF restored alcohol-induced intestinal flora imbalance by maintaining the abundance of intestinal flora and repairing intestinal mucosal barrier damage.Conclusion These findings suggest that PF can effectively ameliorate alcoholic liver disease by exerting antioxidative and anti-inflammatory effects,as well as regulating intestinal flora disorder.These findings highlight the potential of PF for widespread application and further development.
作者
张兴敏
黄月昌
陈柏忠
李倩
吴盼盼
陈文华
吴日辉
李辰
ZHANG Xing-Min;HUANG Yue-Chang;CHEN Bai-Zhong;LI Qian;WU Pan-Pan;CHEN Wen-Hua;WU Ri-Hui;LI Chen(School of Pharmacy and Food Engineering,Wuyi University,Jiangmen 529020,China;International Healthcare Innovation Institute(Jiangmen),Jiangmen 529040,China;Guangdong Xinbaotang Biotechnology Co.,Ltd.,Jiangmen 529100,China;Guangdong Xinbaotang Pharmaceuticals Co.,Ltd.,Jiangmen 529100,China)
出处
《食品安全质量检测学报》
CAS
2024年第8期85-95,共11页
Journal of Food Safety and Quality
基金
广东省科技厅粤港澳大湾区国际科技创新中心建设项目粤澳合作项目(2022A0505020026)
广东省教育厅项目(2021ZDZX4041、2020KZDZX1202)。
关键词
陈皮酵素
酒精性肝损伤
氧化应激
肠道菌群
Pericarpium citri reticulatae fermentation liquor
alcoholic liver disease
oxidative stress
gut microbiota
