摘要
本试验通过乙醇浸提超声波辅助响应面法优化血满草叶中绿原酸提取工艺,并探究其抗氧化活性。通过单因素和响应面试验探讨对血满草叶中绿原酸提取率的影响因素,优化工艺参数。以DPPH和ABTS自由基清除率为指标,评价血满草叶中绿原酸的体外抗氧化能力。结果表明:血满草叶中绿原酸最佳提取工艺为乙醇浓度30%、液料比45:1(mL/g)、超声时间40 min、超声温度70℃,此条件下血满草叶中绿原酸的提取率为2.55%,与模型预测值接近。体外抗氧化活性试验表明,一定浓度范围内,血满草叶中绿原酸有较好的抗氧化作用。绿原酸提取液中,DPPH和ABTS自由基清除的IC50分别为59.7、23.0 mg/L,DPPH和ABTS自由基最大清除率分别为55.3%、61.5%。
The extraction process of chlorogenic acid from the leaves of Sambucus adnata Wall was optimized by response surface methodology with ethanol extraction and ultrasonic assisted in this paper,and its antioxidant activity was explored.The effects on the extraction rate of chlorogenic acid from the leaves of Sambucus adnata Wall were investigated by single factor and response surface methodology,and the process parameters were optimized.Using the free radical scavenging rate of DPPH and ABTS as indicators,the in vitro antioxidant capacity of chlorogenic acid from the leaves of Sambucus adnata Wall was evaluated.The results showed that the optimal extraction process of chlorogenic acid from the leaves of Sambucus adnata Wall was as follows:ethanol concentration of 30%,liquid-material ratio of 45:1(mL/g),ultrasonic time of 40 min,and ultrasonic temperature of 70℃.The extraction rate of chlorogenic acid was 2.55%,which was close to the predicted value of the model.The in vitro antioxidant activity test showed that chlorogenic acid from the leaves of Sambucus adnata Wall had a good antioxidant effect in a certain concentration range.The IC50 of DPPH and ABTS free radical scavenging from extraction solution of chlorogenic acid were 59.7,23.0 mg/L respectively,and the maximum scavenging rates of DPPH and ABTS free radicals were 55.3%and 61.5%,respectively.
作者
郭蒙
姜春红
刘韬
王玉林
勾娜
罗小虎
GUO Meng;JIANG Chunhong;LIU Tao;WANG Yulin;GOU Na;LUO Xiaohu(School of Chemistry and Chemical Engineering,Qiannan Normal University for Nationalities,Duyun,Guizhou Province 558000,China;Fangtuo Biotechnology(Suzhou)Co.Ltd.,Suzhou,Jiangsu Province 215100,China;Guizhou Province College Research Engineering Center of Material Failure and Protection,Duyun,Guizhou Province 558000,China)
出处
《中国饲料》
北大核心
2023年第21期53-60,共8页
China Feed
基金
贵州省教育厅青年科技人才成长项目(黔教合KY字[2018]440)
国家级大学生创新创业训练一般项目(202210670059)
黔南民族师范学院教育质量提升工程项目(2017xjg0402)
贵州省高等学校材料失效与防护工程研究中心(黔教技[2022]057)。
关键词
血满草叶
绿原酸
响应面法
超声波
抗氧化
leaves of Sambucus adnata Wall
chlorogenic acid
response surface methodology
ultrasonic
antioxidant
