摘要
目的 :探讨EGF、bFGF、TGF -β1,及EGF与TGF -β1共同作用对培养鸡角膜缘上皮细胞增殖性的影响。方法 :用DMEM和F12 (1∶1)培养基 ,3 %胎牛血清 ,加入不同浓度EGF、或bFGF、或TGF -β1、或EGF与TGF -β1,采用MTT法检测培养细胞的OD值。结果 :除 1ng/ml组外 ,其余从 5～ 160ng/ml的EGF各浓度组增殖效应均明显大于对照组 (P <0 0 5 ) ,10ng/ml组明显大于其它各浓度组 (P <0 0 2 ) ;bFGF各浓度组 ,除 1ng/ml组和 160ng/ml组外 ,其余从 5～ 80ng/ml各组均明显大于对照组 (P <0 0 2 5 ) ,2 0ng/ml组明显大于与其它各组 (P <0 0 2 5 ) ;TGF -β1各浓度组 ,除 0 1ng/ml组和 0 2ng/ml组外 ,其余从 0 4～12 8ng/ml各组抑制效应明显大于对照组 (P <0 0 2 5 ) ,6 4ng/ml和 12 8ng/ml两组比较未见显著性差异 (P =1 0 0 0 ) ,两组分别与其它各组比较均有显著性差异 (P <0 0 2 5 ) ;EGF加TGF -β1各浓度组 ,除 1ng/ml组和 160ng/ml组外 ,其余从 5～ 80ng/ml各组增殖效应均明显大于对照组 (P <0 0 5 ) ,10ng/ml组明显大于其它各组 (P <0 0 2 5 )。结论 :EGF、bFGF对培养鸡角膜缘上皮细胞有明显促增殖作用 ;TGF -β1则有明显的抑制作用 ;这些作用均呈剂量依赖性 ;EGF与TGF -β1共同表现为促增殖作用。
Objective:To investigate the effect of EGF,bFGF,TGF-β1 and EGF with TGF-β1 on the proliferation of cultured chicken limbal epithelial cell. Methods:Prepare the suspension of limbal epithelial cell with the mixed culture liquid DMEM/F12(1∶1),3% fetal bovine serum,EGF,bFGF,TGF-β1 and EGF with TGF-β1 at different concentration.The value of OD on proliferation of cell was figured out with the MTT pathway. Results:The affection of every group of EGF (the concentration from 5ng/ml to 160ng/ml) was significantly stronger than that of the controls(P<0 05).The group of 10ng/ml was significantly stronger than that of the others(P<0 025).The proliferation affection of bFGF in every group(from 5ng/ml to 80ng/ml)was stronger than that of the controls(P<0.025).The proliferative situation of TGF-β1 in every group(from 0.4ng/ml to 12.8mg/ml) was weaker than that of the controls (P<0 025);between groups of 6.4ng/ml and 12.8ng/ml,there was no significant difference(P=1 000),but comparing with other groups(6 4ng/ml or 12 8ng/ml group vs 0 4ng/ml or so) the differences were all significant(P<0 025).The effect of proliferation of EGF together with TGF-β1 in every group was stronger than that of the controls(P<0 05).The effect of proliferation caused by EGF and TGF-β1 was dose-depended.But beginning after 10ng/ml,the effect decreased with the increase of concentration. Conclusions:The effect of EGF and bFGF strengthening the proliferation of cultured cell and the inhibition of TGF-β1 on the proliferation of cultured cell was found and showed dose-depended manner.The effect of EGF together with TGF-β1 still appeared to promote the proliferation.
出处
《中国实用眼科杂志》
CSCD
北大核心
2002年第11期868-870,共3页
Chinese Journal of Practical Ophthalmology
基金
本课题受广东省卫生厅科研基金资助(A2000637)
