摘要
目的 探讨转化生长因子 β1(TGF β1)对角膜碱烧伤机体巨噬细胞和淋巴细胞功能的影响。 方法 Balb/c鼠单眼角膜Ⅱ度碱烧伤 ,治疗组于模型制作后 1h内球后注射TGF β15 0ng联合腹腔注射TGF β130ng。动态观察腹腔巨噬细胞吞噬功能和循环淋巴细胞对促分裂原刺激的转化功能 ,并与对照组进行对比分析。结果 经TGF β1治疗后 ,腹腔巨噬细胞吞噬能力在 5周内明显低于对照组 (P <0 0 5 ) ;淋巴细胞转化在植物血凝素 (PHA)刺激时 ,3周内显著降低(P <0 0 5 ) ,但在烧伤角膜蛋白刺激时 ,差别无显著性 (P >0 0 5 )。结论 微量TGF β1对角膜碱烧伤机体免疫活性细胞的功能具有抑制作用。
ObjectiveTo observe the effect of transforming growth factor β1(TGF β1)on the immune cells following corneal alkali burns.MethodsBalb/c mice were given mild corneal alkali burns.In treatment group,the mice were injected TGF β1 50?ng postbularly combined with 30?ng abdomino injection.The control mice were given corneal burns without TGF β1 injection.In both groups,the macrophages were obtained by washing the abdominal cavity of mice using normal saline,and the lymphocytes in the blood were acquired at the same time by density gradient centrifugation.The normal corneas(control group)and burned corneas were excised and homogenized in PBS.The homogenate was then centrifuged and the supernatant was used as a source of corneal protein.Every other week in a period of 7 weeks,the macrophages were used for tests of phagocytosis while the lymphocytes were cultured in complete RPMI 1?640 culture medium with or without phytoagglutinin(PHA)or mice corneal protein for 54 h followed by incubation with 3?H TdR for 18 h.Counts per minute(cpm),relative plastid transformation index(RPI)and stimulation index(SI)were analyzed.ResultsFor mice with TGF β1 injection,the phagocytosing rate within 5 weeks and phagocytosing index during 3～5 weeks were obviously lower than those in controls( P <0 05);and the △cpm,RPI or SI in blastogenesis tests induced by PHA before the 3rd week were markedly lower than those of control mice( P <0 05). There was no difference between the two groups in the △cpm,RPI or SI in blastogenesis tests induced by mice corneal proteins during the period of observation( P >0 05).ConclusionTGF β1 can suppress the phagocytic function of macrophages and blastogenesis of lymphocytes induced by PHA.
出处
《眼科研究》
CAS
CSCD
2000年第2期101-103,共3页
Chinese Ophthalmic Research
基金
广东省自然科学基金!资助 (基金编号 :960 2 0 2 )
