摘要
目的 :观测血管紧张素II(AngiotensinII ,AngII)对体外培养的牛眼小梁细胞 (bovinetrabecularmeshworkcells ,TMcell ) 3H胸腺核苷酸 (3H -thymidine,3H -TdR)掺入率的影响 ,探讨原发性开角青光眼 (primaryopen -angleglaucoma ,POAG)的发病机制。方法 :AngII(1× 10 - 7mol·L - 1及 1× 10 - 8mol·L - 1)以及其I型受体 (an giotensinreceptortypeI,AT1)拮抗剂Losartan孵育TM细胞 ,采用检测 3H -TdR掺入率的方法了解细胞增殖状态。结果 :AngII明显的增加了TM细胞的 3H -TdR摄入率 ,Losartan可部分阻断其增殖效应。结论 :AngII可以诱导体外培养的牛眼TM细胞的细胞增殖率升高 。
OBJECTIVE: To investigate the effects of angiotensin II (Ang II) on the cell proliferation of bovine trabecular meshwork (TM) cells in vitro. May probe into the mechanism of primary open-angle glaucoma (POAG).METHODS: Ang II(1×10-7mol·L-1 and 1×10-8mol·L-1)and angiotensin receptor type I(AT1)antagonist (Losartan)were incubated with cultured TM cells. The cellular proliferation was measured by 3H-thymidine(3H-TdR) incorporation assay.RESULTS: Ang II increased the TM cells uptake of 3H-TdR and inhibited by Losartan partially . CONCLUSION: Ang II have the effect that inducing the proliferation of bovine TM cells in vitro. The AT1 antagonist may inhibit this cell proliferation effect.
出处
《航空航天医药》
2002年第1期1-3,共3页
Aerospace Medicine
