摘要
为了提高秋子梨组培幼苗的生根率,本研究以秋子梨生根组培培养基(1/2MS+IBA 0.5 mg/L)为基础,分别添加不同浓度的活性炭(AC):0.25、0.50、0.75、1.00 g/L,分析不同的活性炭含量对秋子梨组培苗生长的影响。结果表明:加入活性炭培养的组培苗在生根率、生根数、根长等方面均显著高于对照组,最适宜秋子梨生根的培养基为1/2MS+IBA 0.5 mg/L+AC 1.00 g/L,该处理的秋子梨组培苗生根率达到了90%,可明显促进秋子梨组培的生长。本研究结果为提高梨属植物组培幼苗的生根率和成活率提供理论依据,同时为木本植物的快繁体系的建立提供参考。
In order to improve the rooting rate of P.ussuriensis tissue culture seedlings,this study added different concentrations of activated carbon(AC)(0.25,0.50,0.75,1.00 g/L)on the P.ussuriensis rooting tissue culture medium(1/2MS+IBA 0.5 mg/L),and analyzed the effects of different activated carbon content on the growth of P.ussuriensis tissue culture seedlings.The results showed that the rooting rate,rooting number and root length of tissue culture seedlings cultured with activated carbon were significantly higher than those of the control group.The most suitable medium for rooting P.ussuriensis was 1/2MS+IBA 0.5 mg/L+AC 1.00 g/L,and the rooting rate of the treated P.ussuriensis tissue culture seedlings reached 90%,which could obviously promote the growth of P.ussuriensis tissue culture.The results provide a theoretical basis for improving the rooting rate and survival rate of tissue culture seedlings of pear plants,and provide a reference for the establishment of rapid propagation system of woody plants.
作者
栾晓龙
史昊
许波
张倩男
刘莉
Luan Xiaolong;Shi Hao;Xu Bo;Zhang Qiannan;Liu Li(School of Horticulture,Anhui Agricultural University,Hefei,230036)
出处
《分子植物育种》
CAS
北大核心
2023年第2期589-593,共5页
Molecular Plant Breeding
基金
安徽省大学生创新创业项目(S202010364243,XJDC2020399)
国家现代农业产业技术体系建设专项(CARS-29-14)共同资助。
