摘要
对梨S系矮化砧试管苗增殖系数和生长状态的影响因子进行了研究 ,结果表明 :S1 ～S6 不同砧木类型试管分化能力存在差异 ;培养基中添加 1 0mg/L的 6 BA ,既能保证试管苗较高的增殖率 ,又能保证试管苗的质量 ;试管苗再生能力随继代培养代数的增加而提高 ;培养基中GA3≤ 0 1mg/L和使用组培专用封口膜 ,可以有效提高试管苗的分化质量 ,防止玻璃苗产生。
The influencing factors of multiplication coefficient and regeneration quality for adventitious shoots of the S series dwarfing rootstock of pear were studied. The results showed: The regeneration capability of the adventitious shoots in different rootstocks was different. The multiplication coefficient and the quality of the shoots were higher on culture media with 1.0 mg/L 6-BA. The more the subculture times were,the higher the regeneration capability was.keeping GA 3 concentration under 0.1 mg/L and use of appropriative sealing material could improve the quality of adventitious shoots and overcome vitrification.
出处
《河北农业科学》
2004年第2期40-44,共5页
Journal of Hebei Agricultural Sciences
基金
河北省科技攻关项目 ( 972 45 5 0 6D)
关键词
梨
S系矮化砧
组培苗
增殖系数
分化质量
继代培养
S series dwarfing rootstock of pear
Adventitious shoots
Multiplication coefficient
Subculture
Regeneration quality
