摘要
西印度醋栗(Phyllanthus acidus)是一种生长于热带地区的乔木,兼具食用和药用价值,开展其组培快繁技术研究,可促进其资源的持续开发与利用。以西印度醋栗茎尖为外植体,对初代培养、继代增殖培养及生根培养方案进行初步筛选。结果表明,初代培养最适培养基为MS+2.0 mg·L^(-1)6-BA+0.2 mg·L^(-1) NAA,外植体诱导萌发率达81.11%;继代增殖最适培养基为MS+1.0 mg·L^(-1)6-BA+0.2 mg·L^(-1) IBA,增殖系数达1.86;最适生根培养基为MS+1.5 mg·L^(-1) IBA,生根率达83.00%;炼苗7天后移栽,移栽基质为珍珠岩:泥炭土:腐殖质=1:1:1(v/v/v),成活率为90%。从接种外植体到获得再生植株约90天。研究结果为西印度醋栗的资源保护与产业化应用提供了技术支持。
INTRODUCTION:A preliminary tissue culture system for Phyllanthus acidus was established.RATIONALE:In this study,the stem tips of P.acidus were used as explants,and the schemes of primary culture,secondary proliferation culture and rooting culture were screened.RESULTS:The results showed that the optimal medium for primary culture was MS+2.0 mg·L^(-1)6-BA+0.2 mg·L^(-1) NAA,and the induced germination rate of explants reached 81.11%.The optimal medium for subculture was MS+1.0 mg·L^(-1)6-BA+0.2 mg·L^(-1) IBA,and the proliferation coefficient was 1.86.The optimal medium for rooting was MS+1.5 mg·L^(-1) IBA,and the rooting rate reached 83.00%.After 7 days of cultivation,the plantlets were transplanted with perlite,peat soil and humus=1:1:1(v/v/v),and the survival rate was 90%.CONCLUSION:The most suitable medium for primary culture using the stem tip of P.acidus as explant was MS medium and the phytohormones were 2.0 mg·L^(-1)6-BA and 0.2 mg·L^(-1) NAA;the optimal phytohormones for subculture were 1.0 mg·L^(-1)6-BA and 0.2 mg·L^(-1) IBA;the optimal phytohormone for rooting culture was 1.5 mg·L^(-1) IBA.
作者
李彤
李楚然
张芷瑜
付晓熳
刘云
张颖君
杨力颖
赵平
Tong Li;Churan Li;Zhiyu Zhang;Xiaoman Fu;Yun Liu;Yingjun Zhang;Liying Yang;Ping Zhao(Key Laboratory of State Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming 650224,China;Key Laboratory of Phytochemistry and Natural Medicines,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China;Yunnan Xinxing Greening Engineering Co.,Ltd.,Kunming 650228,China)
出处
《植物学报》
北大核心
2025年第4期611-620,共10页
Chinese Bulletin of Botany
基金
国家自然科学基金(No.32270426,No.82074124)。
关键词
西印度醋栗
组织培养
快速繁殖
Phyllanthus acidus
tissue culture
rapid propagation
