摘要
目的分析95例医务工作者接种新型冠状病毒(2019-nCoV)灭活疫苗后,血液中免疫抗体结果,探讨新冠灭活疫苗接种后抗体的产生规律和特征。方法收集2020年10月-2021年7月解放军第305医院完成两剂新冠灭活疫苗注射后第30、65、91天、6个月、9个月时95例医务工作者的静脉血样本,采用化学发光免疫分析检测新冠病毒免疫球蛋白(Ig)M、IgG、中和抗体和总抗体滴度,动态分析抗体检测结果,确定抗体免疫持久性及影响因素、相关性等。结果在注射两针新冠灭活疫苗后第30天几乎全部受试者均产生了IgG、中和抗体和总抗体,部分受试者仍保留较高水平的IgM抗体;其中,吸烟可影响总抗体的产生,而低体质量组可产生更多的IgG抗体,当超过60 kg后,无统计学差异;而在接下来的检测时间点,四种抗体的滴度均有下降,至9个月时,除IgG外其他抗体阳性率已不足50%。结论注射两针新冠灭活疫苗后可产生相应的特异性IgM、IgG、中和抗体和总抗体,但随着时间进展,抗体滴度明显降低,抗体阳性率下降,机体保护作用下降。因此,建议接种过两剂新冠灭活疫苗6个月后再次接种加强针疫苗,以提高对新型冠状病毒的自体免疫力。
OBJECTIVE To investigate the immune antibodies in blood specimens of 95 health care workers vaccinated with inactivated 2019-nCoV vaccines and explore the rules and characteristics of production of antibodies after vaccination.METHODS From Oct 2020 to Jul 2021,the venous blood specimens were collected from 95 health care workers of the 305 Hospital of PLA after the injection of 2 doses of 2019-nCoV vaccines fo30 days,65 days,91 days,6 months and 9 months.SARS-CoV-2 immunoglobin(Ig)M,IgG and titers of neutralizing antibodies and total antibodies were detected by chemiluminescence immunoassay,the results of antibody tests were dynamically analyzed,the immune durability of the antibody,influencing factors and correlation were determined.RESULTS Almost all of the subjects produced IgG,neutralizing antibody and total antibody,some subjects retained high level of IgM titer.Smoking could affect the production of total antibody.The subjects of the low body weight group produced higher level of IgG,and there was no significant difference when the weight was over 60 kg.The titers of the four types of antibodies decreased significantly at the following time points,and the positive rates of all the antibodies were less than 50%except for IgG after the vaccination for 9 months.CONCLUSION Specific IgM and IgG,neutralizing antibody and total antibody can be produced after the 2-doses vaccination of inactivated 2019-nCoV vaccines.But the titers and positive rates of the antibodies decrease with time,which means the protective effects on the body decrease.Therefore,in order to improve the autoimmunity against novel coronavirus,one booster vaccination of an inactivated 2019-nCoV vaccine will be necessary after the 2 doses of vaccination for 6 months.
作者
刘梦颖
梁雪梅
林虎
华翔
江继平
蒋知新
曾刚
祝丙华
张金萍
LIU Meng-ying;LIANG Xue-mei;LIN Hu;HUA Xiang;JIANG Ji-ping;JIANG Zhi-xin;ZENG Gang;ZHU Bing-hua;ZHANG Jin-ping(The 305 Hospital of PLA,Beijing 100017,China;不详)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2022年第22期3497-3501,共5页
Chinese Journal of Nosocomiology
基金
国家重点研发计划基金资助项目(2021YFC2302004)。
关键词
新型冠状病毒
灭活疫苗
抗体
免疫持久性
影响因素
SARS-CoV-2
Inactivated vaccine
Antibody
Immune durability
Influencing factor
