摘要
目的:使用多种鉴定方法联合鉴定1株实验室未知菌株,并利用葡聚糖硫酸钠诱导的实验性小鼠结肠炎模型,评价其缓解炎症性肠病的功效。方法:利用16S rRNA和保守蛋白编码基因gyrA及gyrB进行初步鉴定,通过毒力基因nheA、nheB、nheC、hblA、hblC、hblD、becT、cytK、entFM及其生化反应特性确定种属,并测定分离菌在不同温度、pH值以及人工胃肠液的耐受特性。进一步建立实验性结肠炎小鼠模型,探究其缓解炎症性肠病的潜在功效。结果:分子及生理生化鉴定结果显示分离菌为蜡样芽孢杆菌(Bacillus cereus),并命名为HMPM18123。生长特性测定结果还显示,该菌株具有良好的耐高温和胃液肠液耐受特性。此外,该菌株具有改善实验性小鼠结肠炎的功效,具体表现为缓解小鼠结肠炎导致的体质量减低、结肠缩短、疾病活动指数评分升高、组织病变和炎症因子的表达水平上调。
An unknown laboratory strain was identified and named as Bacillus cereus HMPM18123 based on the 16S rRNA gene and conserved protein-coding gene(gyrA and gyrB)sequences combined with virulence genes(nheA,nheB,nheC,hblA,hblC,hblD,becT,cytK and entFM)and biochemical properties.The tolerance of the strain to different temperatures,pH,simulated gastric and intestinal fluid was evaluated as well as its efficacy in alleviating colitis induced by dextran sulfate sodium(DSS)in mice.The results of growth characteristics showed that the strain had good tolerance to high temperature and simulated gastric and intestinal fluid.In addition,the strain improved DSS-induced colitis symptoms in mice such as body mass loss,colon shortening,increased disease activity index(DAI)score,tissue lesions,and up-regulated expression of inflammatory factors.
作者
许一凡
盛康亮
王永中
XU Yifan;SHENG Kangliang;WANG Yongzhong(Key Laboratory of Human Microecology and Precision Medicine of Anhui Province,College of Life Sciences,Anhui University,Hefei 230601,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2023年第2期173-180,共8页
Food Science
基金
国家自然科学基金青年科学基金项目(32000081)
国家自然科学基金面上项目(31770066)
安徽省自然科学基金项目(1908085QC120)
安徽大学博士科研启动基金项目(J01003316)。
关键词
蜡样芽孢杆菌
保守蛋白编码基因
毒力基因
生长特性
炎症性肠病
Bacillus cereus
conserved protein-coding genes
virulence genes
growth characteristics
inflammatory bowel disease
