摘要
水稻作为全球主要的粮食作物之一,每年因稻瘟病危害所造成的减产可高达10%~30%,而利用水稻抗病基因通过分子标记辅助育种选择技术(marker assistant aelection,MAS),可以安全有效且精准快速地定向培育含抗稻瘟病基因的抗性品种。稻瘟病抗病基因Pi-kh已被证明对稻瘟病菌具有广谱抗性,因此通过MAS对Pi-kh基因加以选择,并在水稻抗病育种中加以利用,对提高水稻稻瘟病抗性具有较高的应用价值。本研究基于Pi-kh基因编码序列区(coding sequence,CDS)的两个缺失(Insert delete,InDel)差异,在InDel两端的保守序列位置开发了1个共显性功能分子标记,将其命名为ZJPikh。并将该标记用于以携带Pi-kh感病基因型品种‘玉稻518’为母本,Pi-kh抗病基因型品系‘师大68’为父本的抗病品种选育中,通过系谱法与MAS并对F2分离群体及高世代植株稻瘟病关联基因Pi-kh抗病基因型进行选择,成功选育出了携带有Pi-kh的稻瘟病抗性达三级的中抗稻瘟病新品系‘玉稻58’,同时准确高效地鉴定了21份水稻品种或品系的Pi-kh基因型,为通过MAS技术定向高效培育携带Pi-kh基因的抗稻瘟病品种提供技术支撑。本研究为筛选稻瘟病抗性种质资源及利用分子标记辅助抗病育种提供了依据。
Rice,as one of the staple food crops in the world,is reduced by 10%~30%every year due to the damage of rice blast.However,the application of marker assistant selection(MAS)with rice disease resistance genes can be effectively,accurately and rapidly used to breed rice blast resistant varieties.The rice blast resistance gene Pi-kh has been proved to have broad-spectrum resistance to the rice blast.Therefore,the selection and utilization of Pi-kh gene by MAS in rice disease resistance breeding possess high application value for improving rice blast resistance.In this study,based on two InDel differences in coding sequence(CDS)region of Pi-kh gene,a co-dominant functional molecular marker was developed at the conservative position of the sequence,which was named ZJPikh.The marker was used in the disease resistance breeding with rice blast susceptible variety'Yudao 518'as female parent and rice blast resistant variety'Shida 68'as male parent.The genotype of gene Pi-kh related with rice blast resistance in F2 isolated population and high generation plants was verified by pedigree method and MAS.A new rice blast resistant third-grade variety'Yudao 58'with Pi-kh was successfully bred.At the same time,the Pi-kh genotypes of 21 main rice varieties were identified,which provided technical support for the targeted and efficient breeding of blast resistant varieties carrying Pi-kh gene through MAS technology.Our research provides convenience for identifying rice blast resistance germplasm resources and molecular marker-assisted disease resistance breeding.
作者
桑世飞
王君怡
周静
曹梦雨
王亚男
张建强
姬生栋
张文玲
Sang Shifei;Wang Junyi;Zhou Jing;Cao Mengyu;Wang Yanan;Zhang Jianqiang;Ji Shengdong;Zhang Wenling(College of Life Sciences,Henan Normal University,Xinxiang,453007;Henan Seed Management Station,Zhengzhou,450046)
出处
《分子植物育种》
CAS
北大核心
2022年第5期1588-1596,共9页
Molecular Plant Breeding
基金
国家重点研发计划专项(2018YFD0200200)
河南水稻产业体系项目(Z2012-04-G02)
河南省重大科技专项(171100110300-4)共同资助。
