摘要
以秦皮为原料,香豆素得率为考察指标,在单因素试验的基础上,采用正交试验优化超声辅助法提取秦皮中香豆素的工艺条件。结果表明,最佳提取工艺条件为:表面活性剂用量3%,乙醇体积分数50%,提取温度60℃,提取时间40 min,料液比1∶10(g/mL),在此工艺条件下,香豆素得率高达1.038%。对提取物进行抗氧化性试验,结果表明,相同浓度下香豆素提取液对DPPH自由基和羟自由基的清除能力均强于VC,秦皮中香豆素具有较强的抗氧化性。
In this study,the ultrasonic-assisted extraction technology conditions of coumarin from cortex fraxini were optimized by orthogonal experiment on the basis of single factors tests using cortex fraxini as the raw material and coumarin extraction yield as the indicator.Results showed that the optimal extraction conditions were determined as follows:surfactant addition of 3%,ethanol volume fraction of 50%,extraction temperature of 60℃,extraction time of 40 min,and solid-liquid ratio of 1∶10(g/mL).Under these conditions,the extraction yield of coumarin reached to 1.038%.Subsequently,the antioxidant activity determination results showed that the scavenging capacities of cortex fraxini extract on DPPH and hydroxyl free radicals were both higher than that of VC under the same concentrations,which indicated that the coumarin in cortex fraxini had strong antioxidant activity.
作者
童天娇
刘伟
胥鑫萌
李恒睿
刘军海
TONG Tian-jiao;LIU Wei;XU Xin-meng;LI Heng-rui;LIU Jun-hai(College of Chemistry&Environmental Science of Shaanxi University of Technology,Hanzhong 723000,China)
出处
《保鲜与加工》
CAS
2021年第12期46-50,58,共6页
Storage and Process
关键词
秦皮
香豆素
提取
表面活性剂
抗氧化性
cortex fraxini
coumarin
extraction
surfactant
antioxidant activity
