摘要
本试验旨在研究在脱氧雪腐镰刀菌烯醇(DON)污染的饲粮中添加植物炭黑对断奶仔猪生长性能、血清抗氧化指标和肠道二糖酶活性的影响。试验选取48头24日龄、体重为(7.76±0.24)kg、健康的“杜×大×长”断奶仔猪(公猪),随机分为4组,每组3个重复,每个重复4头猪。4组分别为:对照组,饲喂基础饲粮;植物炭黑组,饲喂基础饲粮+0.1%植物炭黑;DON组,饲喂基础饲粮+1.5 mg/kg DON;DON+植物炭黑组,饲喂基础饲粮+1.5 mg/kg DON+0.1%植物炭黑。试验期为21 d。结果表明:1)饲粮添加DON显著降低断奶仔猪末重及第11~21天和第1~21天平均日增重(ADG)(P<0.05),显著降低第10天血液白细胞计数(WBC)、血红蛋白(HGB)含量以及血清总抗氧化能力(T-AOC)和过氧化氢酶活性(P<0.05),显著降低第21天血液WBC、HGB含量以及血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶活性和T-AOC(P<0.05),显著提高第10天和第21天血清丙二醛(MDA)含量(P<0.05),并引起十二指肠蔗糖酶活性以及空肠麦芽糖酶和蔗糖酶活性的异常提高(P<0.05)。2)与DON组相比,在DON污染的饲粮中添加0.1%植物炭黑显著提高了断奶仔猪第1~10天平均日采食量以及第21天血清SOD活性(P<0.05),并且能缓解DON引起的仔猪末重、第11~21天和第1~21天ADG、第10天红细胞计数以及第21天WBC的降低,抑制第10天和第21天血清MDA含量以及空肠蔗糖酶活性的异常升高,并且各指标与对照组相比均无显著差异(P>0.05)。3)正常饲粮中添加植物炭黑对断奶仔猪生长性能、血常规指标、血清抗氧化指标和小肠二糖酶活性均无负面影响(P>0.05)。综上所述,在本试验条件下,饲粮添加0.1%植物炭黑能缓解DON引起的断奶仔猪生长性能和血清抗氧化能力的下降,抑制DON造成的空肠蔗糖酶活性的异常升高。
The present study was conducted to investigate the effects of dietary plant carbon black on growth performance,serum antioxidant indices and intestinal disaccharidase activity of weaned piglets fed deoxynivalenol contaminated diets.Forty-eight healthy Duroc×Yorkshire×Landrace weaned piglets(boars)with body weight of(7.76±0.24)kg at the age of 24 days were randomly divided into 4 groups with 3 replicates per group and 4 pigs per replicate.The four groups were as follows:control group,fed a basal diet;plant carbon black group,fed the basal diet+0.1%plant carbon black;DON group,fed the basal diet+1.5 mg/kg DON;DON+plant carbon black group,fed the basal diet+1.5 mg/kg DON+0.1%plant carbon black.The experiment lasted for 21 days.The results showed as follows:1)dietary DON significantly decreased the final body weight and average daily gain(ADG)of weaned piglets from days 11 to 21 and days 1 to 21(P<0.05),significantly decreased the white blood cell count(WBC)and hemoglobin(HGB)content in blood,as well as the total antioxidant capacity(T-AOC)and catalase activity in serum on day 10(P<0.05),significantly decreased the WBC and HGB content in blood and the activity of superoxide dismutase(SOD)and glutathione peroxidase as well as the T-AOC in serum on day 21(P<0.05),significantly increased the malondialdehyde(MDA)content in serum on days 10 and 21(P<0.05),and abnormally increased the sucrase activity in duodenum and the activity of maltase and sucrase in jejunum(P<0.05).2)Compared with the DON group,dietary 0.1%plant carbon black in the DON contaminated diet significantly increased the average daily feed intake from days 1 to 10 and the serum SOD activity on day 21 of weaned piglets(P<0.05),alleviated the decrease of final weight,ADG from days 11 to 21 and days 1 to 21,red blood cell count on day 10 and WBC on day 21 of piglets induced by DON,inhibited the abnormal increase of serum MDA content and jejunal sucrase activity on days 10 and 21,and there was no significant difference in each index compared with the control group(P>0.05).3)Dietary plant carbon black in normal diet had no negative effects on growth performance,blood routine indices,serum antioxidant indices and disaccharidase activity in small intestine of weaned piglets(P>0.05).In conclusion,under the conditions of this study,dietary 0.1%plant carbon black can alleviate the decrease of growth performance and serum antioxidant capacity of weaned piglets,and inhibit the abnormal increase of jejunal sucrase activity induced by DON.
作者
吴杰
邓波
陈雪颖
王翰阳
徐子伟
WU Jie;DENG Bo;CHEN Xueying;WANG Hanyang;XU Ziwei(Institute of Animal Husbandry and Veterinary Science,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;College of Animal Science and Technology,Northeast Agriculture University,Harbin 150000,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2021年第10期5917-5926,共10页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
浙江省重点研发项目(No.2021C02007C)
现代农业产业技术体系建设专项基金(CARS-35)
浙江省畜禽育种专项(2016C02054-4,2016C02054-7)。
