摘要
基于网络药理学的方法探讨天南星(Arisaema consanguineum)的抗癌机制。利用TCMSP数据库、GeneCards数据库、Uniprot数据库查找天南星的活性成分和筛选抗癌靶点。通过Cytoscape3.7.1软件、STRING数据库、DAVID数据库、ImageGP软件建立化合物—抗癌靶点可视化网络图和抗癌靶点互作图,对靶点生物过程和通路进行分析。结果表明,共收集天南星主要抗癌活性成分松油醇、亚油酸等42个,作用于ADH1B、ADH1C等65个抗癌靶点,参与活性氧合成过程、腺体形态发生、急性炎症反应调控、细胞增殖等主要生物过程,调控神经活性配体—受体相互作用通路、癌症通路、P53信号通路、TNF信号通路、结直肠癌通路、小细胞肺癌通路、非小细胞肺癌通路、甲状腺癌通路、前列腺癌通路、细胞凋亡等通路,为今后天南星抗癌机制的深入研究提供了理论依据。
This study used the method of network pharmacology to study the anticancer mechanism of Arisaema consanguineum.The active components and targets of Arisaema consanguineum were searched using the TCMSP database,and these targets were uniformly named using the Uniprot database.Cytoscape 3.7.1 was used to establish a compound-anti-cancer target visualization network map,and the STRING database was used to construct the PPI network.Enrichment pathway analysis was performed using the DAVID web⁃site and the ImageGP database.Bioprocess analysis was performed using ClueGo software.The results showed that,a total of 42 anticancer active components and 65 anti-cancer targets were collected.The main anticancer active substances of Arisaema consanguine⁃um include terpineol and linoleic acid.Important anti-cancer targets are ADH1B,ADH1C and the like.Arisaema consanguineum can show the multi-component,multi-target and multi-pathway anticancer function by by regulating TNF signaling pathways and p53 sig⁃naling pathways through biological processes such as participation in neuroactive ligand-receptor interactions and cancer processes.
作者
郝爱平
吴启康
国会艳
薛巨坤
魏继承
任如意
HAO Ai-ping;WU Qi-kang;GUO Hui-yan;XUE Ju-kun;WEI Ji-cheng;REN Ru-yi(Mudanjiang Normal University,Mudanjiang 157011,Heilongjiang,China)
出处
《湖北农业科学》
2020年第23期110-115,142,共7页
Hubei Agricultural Sciences
基金
黑龙江省省属高等学校基本科研业务费科研项目(1353MSYYB011)
黑龙江省大学生创新创业训练计划项目(201810233033)。
