摘要
目的探讨核苷酸切除修复交叉互补基因(ERCCl)、3型微管蛋白编码基因(TUBB3)、拓扑异构酶Ⅱα(TOPOⅡα)在小儿肾母细胞瘤组织中的表达情况及其与临床病理特征的相关性。方法选取2017年9月至2019年8月在郑州大学第一附属医院进行治疗的42例小儿肾母细胞瘤患儿的临床资料,将其肾母细胞瘤组织标本作为A组,将癌旁组织标本作为B组,检测TUBB3、ERCC1、TOPOⅡα在两组组织中的表达情况,并分析其与临床病理特征的关系。结果A组标本的TUBB3、ERCC1、TOPOⅡα高表达率[47.62%(20/42)、40.48%(17/42)、59.52%(25/42)]明显高于B组[7.14%(3/42)、4.76%(2/42)、14.29%(6/42)],差异有统计学意义(P<0.05);预后良好患儿的ERCC1高表达比例明显低于预后不良患儿(P<0.05);Ⅰ~Ⅱ期患儿的ERCC1、TOPOⅡα高表达比例低于Ⅲ~Ⅳ期患儿(P<0.05)。结论肾母细胞瘤组织标本的TUBB3、ERCC1、TOPOⅡα表达明显高于癌旁组织,且ERCC1、TOPOⅡα高表达与病理分期、预后具有相关性。
Objective To investigate the expression of TUBB3,ERCC1,and TOPOIIαin pediatric nephroblastoma and their correlation with clinicopathological characteristics.Methods Fortytwo children with pediatric nephroblastoma treated in the First Affiliated Hospital of Zhengzhou University from September 2017 to August 2019 were selected as the group A and the adjacent tissue samples were taken as group B.The expression of TUBB3,ERCC1,and TOPOIIαwas detected in the two groups and their relationship with clinicopathological features was analyzed.Results The high expression rates of TUBB3,ERCC1,and TOPOⅡαin group A[47.62%(20/42),40.48%(17/42),59.52%(25/42)]were significantly higher than those in group B[7.14%(3/42),4.76%(2/42),14.29%(6/42)](P<0.05);the proportion of high expression of ERCC1 in children with good prognosis was significantly lower than that in children with poor prognosis(P<0.05).The proportion of high expression of ERCC1 and TOPOⅡαin children at stageⅠ-Ⅱat the stage was lower than those in children at stageⅢ-Ⅳ(P<0.05).Conclusions nephroblastoma tissue specimens are significantly higher than those in adjacent tissues,and the high expression of ERCC1 and TOPOⅡαis correlated with pathological stage and prognosis.
作者
苟丽
魏佳丽
杨媛
Gou Li;Wei Jiali;Yang Yuan(Children’s Hospital,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China;Department of Pediatric Surgery,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)
出处
《临床医学》
CAS
2020年第5期10-12,共3页
Clinical Medicine
关键词
肾母细胞瘤
基因表达
病理特征
Nephroblastoma
Gene expression
Pathological features
