肾母细胞瘤组织中TGF-β和IGF-1信号通路表达与细胞增殖活力及侵袭活力的相关性被引量：1

The correlation of TGF-β and IGF-1 signaling pathway expression in nephroblastoma tissue with cell proliferation activity and invasion activity

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摘要目的:研究肾母细胞瘤组织中转化生长因子β(TGF-β)和胰岛素样生长因子-1(IGF-1)信号通路表达与细胞增殖活力及侵袭活力的相关性。方法:选择在武汉大学人民医院手术切除的肾母细胞瘤肿瘤组织及瘤旁组织,抽提蛋白后测定TGF-β和IGF-1信号通路分子的表达量,抽提RNA后测定细胞增殖基因、侵袭基因的mRNA表达量。结果:肾母细胞瘤肿瘤组织中TGFBR1、IGF1R、p-ERK、Smad2/3、p-AKT的蛋白表达量均显著高于瘤旁组织(P<0.05);肾母细胞瘤肿瘤组织中DNp73、Survivin、Ki-67、TUBB3、MMP2、N-cadherin的mRNA表达量均显著高于瘤旁组织,且与TGFBR1、IGF1R、pERK、Smad2/3、p-AKT的蛋白表达量呈正相关;Bmi、TAp73、TIMP1、TIMP2、E-cadherin的mRNA表达量显著低于瘤旁组织(P<0.05),且与TGFBR1、IGF1R、p-ERK、Smad2/3、p-AKT的蛋白表达量呈负相关。结论:肾母细胞瘤组织中TGF-β和IGF-1信号通路的高表达能够促进肿瘤细胞的增殖和侵袭。 Objective:To study the correlation of TGF-βand IGF-1signaling pathway expression in nephroblastoma tissue with cell proliferation activity and invasion activity.Methods:Nephroblastoma tumor tissue and adjacent tissue surgically removed in Renmin Hospital of Wuhan University between May2014and March2017were selected,the expression levels of TGF-βand IGF-1signal pathway molecules were determined after protein extraction,and the mRNA expression levels of cell proliferation genes and invasion genes were determined after RNA extraction.Results:TGFBR1,IGF1R,p-ERK,Smad2/3and p-AKT protein expression levels in nephroblastoma tumor tissue were significantly higher than those in adjacent tissue;DNp73,Survivin,Ki-67,TUBB3,MMP2and N-cadherin mRNA expression levels in nephroblastoma tumor tissue were significantly higher than those in adjacent tissue and positively correlated with TGFBR1,IGF1R,p-ERK,Smad2/3and p-AKT protein expression levels while Bmi,TAp73,TIMP1,TIMP2and E-cadherin mRNA expression levels were significantly lower than those in adjacent tissue and negatively correlated with TGFBR1,IGF1R,p-ERK,Smad2/3and p-AKT protein expression levels.Conclusion:The high expression of TGF-βand IGF-1signaling pathways in nephroblastoma tissue can promote the proliferation and invasion of tumor cells.

作者宋双生徐之良 SONG Shuang-sheng;XU Zhi-liang(Department of Pediatrics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China)

机构地区武汉大学人民医院儿科

出处《海南医学院学报》 CAS 2017年第24期3419-3421,3425,共4页 Journal of Hainan Medical University

基金湖北省自然科学基金资助项目(2013CFB244)~~

关键词肾母细胞瘤转化生长因子Β 胰岛素样生长因子-1 增殖侵袭 Nephroblastoma transforming growth factor β insulin-like growth factor-1 proliferation invasion

分类号 R737.1 [医药卫生—肿瘤]

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1刘卓,何峰,李媛媛,欧阳胜荣,曹丁丁,常会波,马斐斐,吴建新.TGF-β和IGF-1信号通路调控肾母细胞瘤细胞增殖机制探讨[J].中华肿瘤防治杂志,2017,24(12):813-816. 被引量：7
2潘伟康,余辉,王怀杰,郑百俊,郭青青,郭正团,高亚,李鹏.肾母细胞瘤中ERCC1、TUBB3、TOP2A mRNA的表达及临床意义[J].西安交通大学学报（医学版）,2016,37(5):689-692. 被引量：9

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