摘要
本研究考察了粗毛纤孔菌对无机硒的耐受度,并通过响应面法对富硒粗毛纤孔菌菌丝生长培养基成分进行了优化。以粗毛纤孔菌菌丝生长速度为指标,通过单因素实验及Box-Behnken响应面优化,确定该菌菌丝富硒培养基的最佳组成成分,并通过电感耦合等离子体质谱法(ICP-MS)对该菌菌丝硒富集量进行检测。结果表明,粗毛纤孔菌菌丝的生长情况受外源硒影响较大,当硒浓度≥37.25 mg/L时,该菌菌丝生长开始受到显著的抑制(P<0.05),其对硒的最大耐受浓度约为298~596 mg/L;经响应面法优化得到富硒粗毛纤孔菌菌丝生长最优培养基成分为:麸皮56.8 g/L、葡萄糖49 g/L、Se 22.4 mg/L、MgSO40.75 g/L、KH2PO41 g/L、VB11 mg/L、琼脂15 g/L,在此条件下,富硒粗毛纤孔菌实际菌丝生长速度达到8.41 mm/d,与未优化对照组相比,菌丝生长速度提高了0.965倍,且硒富集量达到31.584μg/g。本研究获得了富硒粗毛纤孔菌菌丝生长培养基最优配方,为富硒粗毛纤孔菌菌丝体的大量制备、富硒粗毛纤孔菌子实体的栽培及其它后续研究提供了理论依据。
In this study,the tolerance level of I.hispidus to inorganic Se was investigated.And the culture mediumfor mycelial growth of Se-enriched I.hispidus was optimized using response surface method(RSM).To determine the optimal components of the Se-rich medium,the effect of single factor to mycelial growth of I.hispidus(average mycelium growth rate was calculated as measurement index)was initially identified respectively.Box-Behnken design was then used to create a model for optimizing the Se-rich medium components.Meanwhile,Se content in the mycelia of Se-enriched I.hispidus was detected by ICP-MS.The results showed that the mycelial growth of I.hispidus was greatly influenced by the concentration of exogenous Se.The growth rate of I.hispidus began to be inhibited significantly at 37.25 mg/L Se concentration(P<0.05).And the maximal tolerant concentration of I.hispidus to Se was approximately at 298~596 mg/L.The suitable Se concentration,principal component and supplementary carbon source were selected and adjusted by the model,which predicted the significant effect could be obtained when the Se-rich medium contained bran,glucose,Se,MgSO+4,KH+2 PO4,VB1 and agar at 56.8,49,2.24×10-2,0.75,1,1×10-3 and 15 g/L,respectively.Verification of this recipe resulted in the average mycelium growth rate at 8.41 mm/d,which was 0.965 times higher than that cultivated by using the modified PDA medium with equivalent Se addition.And Se content in the mycelia of Se-enriched I.hispidus reached 31.584μg/g.In this study,the optimal recipe of medium for mycelial growth of Se-enriched I.hispidus was obtained,which provided a basic information for producing mycelia and fruiting bodies of selenized I.hispidus or other subsequent studies.
作者
宋飞飞
沈丽宫
尤洁
王翠
曹晓
王倩雯
SONG Fei-fei;SHEN Li-gong;YOU Jie;WANG Cui;CAO Xiao;WANG Qian-wen(Fujian Vocational College of Bioengineering,Fuzhou 350007,China)
出处
《食品工业科技》
CAS
北大核心
2020年第15期198-204,224,共8页
Science and Technology of Food Industry
基金
福建省教育厅中青年教师科技项目(JAT160759)。
