摘要
以菌丝体生物量、菌丝生长速度和菌丝体胞外多糖含量为指标,对3个雁北嗜蓝孢孔菌菌株液体发酵培养的碳源、氮源和无机盐进行优化筛选,以为该菌的大规模培养和人工驯化提供依据。结果显示,海藻糖是1号和2号菌株的最佳碳源,葡萄糖是3号菌株的最佳碳源;3个菌株的最佳氮源均为豆粕粉;最佳无机盐1号菌株是硫酸镁,2号、3号菌株是磷酸二氢钾。
The mycelium biomass,mycelial activity and extracellular polysaccharide content were used as indicators to study the liquid culture medium of Fomitiporia yanbeiensis collected from three regions,and the best carbon source,nitrogen source and inorganic salt for liquid culture of different strains were screened.The results showed that trehalose was the best carbon source for strain 1 and 2,glucose was the best carbon source for strains 3;the best nitrogen source for all strains was soybean meal;the best inorganic salt for strains 1 was MgSO4,and the best inorganic salt for strains 2 and 3 was KH2PO4.This study can provide the basis for large-scale culture and artificial domestication of this strain.
作者
郭霄飞
李艳婷
郭尚
宋敏丽
白倩
徐莉娜
南晓洁
张程
Guo Xiaofei;Li Yanting;Guo Shang;Song Minli;Bai Qian;Xu Lina;Nan Xiaojie;Zhang Cheng(Shanxi Academy of Agricultural Sciences,Institute of Edible Fungi,Taiyuan,Shanxi 030031,China;Department of Biology in Taiyuan Normal University,Jinzhong,Shanxi 030619,China;School of Life Science,Beijing institute of technology,Beijing 100081,China)
出处
《食药用菌》
2020年第4期272-276,共5页
Edible and Medicinal Mushrooms
基金
山西省科技基础条件平台项目(201705D121012),项目名称为山西省食用菌种质资源共享服务平台建设
山西省农业科学院生物育种工程项目(17yzgc115),项目名称为功能性多孔菌优良菌株的筛选
山西省重点研发计划项目(201803D221009-2),项目名称为特色食药用菌功能产品制造关键技术研究与新产品创制。
关键词
雁北嗜蓝孢孔菌
液体发酵
碳源
氮源
无机盐
Fomitiporia yanbeiensis
liquid culture
carbon source
nitrogen sources
inorganic salts
