摘要
目的探究黄芪甲苷介导TLR4-p38 MAPK信号通路在幼鼠急性肺损伤模型中的作用。方法以7日龄SD新生仔鼠为实验对象,利用脂多糖构建幼鼠急性肺损伤模型,以地塞米松为阳性药对照,每只幼鼠腹腔注射10 m L/kg黄芪甲苷,造模后12 h和24 h观察肺组织病变,检测TLR4、p-p38蛋白和mRNA表达量,TNF-α、IL-6、IL-12炎症因子的mRNA表达量变化。结果模型组在建模后12 h呈现肺出血,大量肺泡萎陷,24 h呈现肺水肿,肺泡间隔明显增宽。和模型组相比,黄芪甲苷组肺组织的病变明显减轻。另外,蛋白检测结果表明,与模型组比较,黄芪甲苷组TLR4、p-p38蛋白表达量明显降低(P<0. 05,P<0. 001) q-PCR结果表明,与模型组比较,黄芪甲苷组TLR4 mRNA相对表达量均有明显的降低,并且和模型组有显著性差异(P<0. 05)。TNF-α、IL-6、IL-12各炎症因子均有下降的趋势,其中IL-12相对表达量和模型组有显著性差异(P<0. 05)。结论黄芪甲苷通过抑制TLR4-p38MAPK信号通路,在幼鼠急性肺损伤模型中起重要作用。
Objective To investigate the effect of astragaloside on the TLR4-p38 MAPK signaling pathway in a newborn mouse acute lung injury model.Methods Seven-day-old SD newborn rats were used as experimental subjects.The model of acute lung injury in newborn rats was established by intraperitoneal lipopolysaccharide(LPS)injection.Dexamethasone was used as a positive control.Each rat was injected intraperitoneally with 10 mL/kg astragaloside.The lung lesions were observed 12 and 24 h after LPS injection.Furthermore,the mRNA and protein expression levels of TLR4 were determined,as well as the phosphorylation level of p38 protein.Finally,the changes in inflammatory factors TNF-α,IL-6 and IL-12 were analyzed.Results The model group showed pulmonary hemorrhage and a large number of collapsed alveoli at 12 h,and pulmonary edema and a significantly widened alveolar septum at 24 h after LPS injection.Compared with the model group,the lesions in the lung tissue of the astragaloside group were significantly alleviated.Additionally,Western blot result showed the levels of TLR4(P<0.05)and p-p38(P<0.001)were significantly lower in the astragaloside group than in the model group.The qPCR result showed that the level of TLR4 mRNA in the astragaloside group was significantly decreased compared with that in the model group(P<0.05).The inflammatory factors,TNF-α,IL-6 and IL-12,were all decreased,and the relative IL-12 expression was significantly different from that in the model group(P<0.05).Conclusions Astragaloside plays an important role in alleviating the acute lung injury model of newborn rats by inhibiting the TLR4-p38 MAPK signaling pathway.
作者
王茜
万静
谭扬茗
罗丹丹
熊海英
姜红
WANG Qian;WAN Jing;TAN Yangming;LUO Dandan;XIONG Haiying;JIANG Hong(Department of Paediatrics,the Central Hospital of Wuhan Affiliated to Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430014,China)
出处
《中国比较医学杂志》
CAS
北大核心
2020年第6期106-112,共7页
Chinese Journal of Comparative Medicine
