摘要
目的:观察厚朴酚(magnolol,Mag)对大鼠急性坏死性胰腺炎(ANP)肺组织中核因子-kB(NF-kB)、白介素-10(IL-10)和肿瘤坏死因子(TNF-a)表达的影响,以探讨厚朴酚对ANP急性肺损伤的保护作用.方法:以50g/L牛磺胆酸钠逆行胆胰管注射制备大鼠ANP模型,SD大鼠56只随机分为假手术(SO)组,ANP模型组,及Mag治疗组.以荧光定量PCR检测不同时间(6,12,24h)肺组织中NF-kB、TNF-a和IL-10的基因表达,同时检测各组血清淀粉酶(Amy)的水平,并观察胰腺和肺的组织病理学改变.结果:与SO组相比,ANP组肺组织中的NF-kB,TNF-a,IL-10mRNA表达量及血清Amy显著增加(P<0.01),其中NF-kB,TNF-amRNA及Amy以12h的升高最明显(P<0.01),IL-10mRNA随ANP时间的进展表达逐渐增加(6,12,24h分别为0.20±0.05,0.27±0.07,0.45±0.20).与ANP各组比较,Mag各组NF-kBmRNA,TNF-amRNA及Amy表达量下降,也以12h下降最显著(分别为1.30±0.14vs1.84±0.56,0.41±0.19vs0.72±0.36,P<0.05;104576±24886nkat/Lvs188621±23747nkat/L,P<0.01),IL-10mRNA的改变无统计学意义(P>0.05).Mag治疗组肺组织学改变较ANP组明显减轻,胰腺组织学损害无明显改善.结论:NF-kB和TNF-a是ANP发生急性肺损伤的重要炎症因子,Mag能抑制其在ANP大鼠肺组织中的表达,对ANP合并急性肺损伤有一定的治疗作用.
AIM： To investigate the protective role of magnolol in the treatment of acute lung injury induced by acute necrotizing pancreatitis （ANP） in rats.
METHODS： Fifty-six Sprague-Dawley rats were divided into sham operation group, ANP group, and magnolol treatment group. ANP model was induced by retrograde infusion of 50 g/L sodium taurocholate into the biliopancreatic duct of rats. The mRNA expression of nuclear factorkappa B （NF-kB）, tumor necrosis factor-alpha （TNF-α）, and interleukin-10 （IL-10） in lung tissue were assayed using fluorescence quantitative polymerase chain reaction （FQ-PCR） at 6, 12, and 24 h. Meanwhile, the activity of serum amylase （Amy） was also determined, and the pathological changes of the pancreas and lung were observed.
RESULTS： In comparison with those in sham operation group, the expression of NF-KB, TNF-o~, and IL-10 mRNA and the activity of se- rum Amy in ANP group were increased signifi- cantly （P 〈 0.01）, and the expression of NF-kB, TNF-α mRNA and the activity of Amy reached the peak at 12 h. The expression of IL-10 mRNA increased gradually at 6,12, and 24 h （0.20±0.05, 0.27±0.07, 0.45± 0.20, respectively）. Magnolol treatment resulted in lower expression of NF- KB, TNF-α mRNA and serum Amy levels （1.30± 0.14 vs 1.84± 0.56, 0.41± 0.19 vs 0.72 ± 0.36, both P 〈 0.05; 104 576±24 886 nkat/L vs 188 621±23 747 nkat/L, P 〈 0.01, respectively, at 12 h）, but didn＇t alter the expression of IL-10 mRNA （P 〉 0.05） as compared with that in ANP group. Magnolol treatment relieved the pathological changes markedly in the lung tissues but not in the pancreatic tissues. CONCLUSION： Magnolol can decrease the expression of the proinflammatory factors such as NF-kB and TNF-ot and relieve the acute lung injury induced by acute necrotizing pancreatitis in rats.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第12期1167-1171,共5页
World Chinese Journal of Digestology
