摘要
目的探究白介素-2(IL-2)及其白介素-2受体(IL-2R)在骨髓间充质干细胞(MSCs)诱导肾移植免疫耐受中对Fas/FasL的表达及T细胞凋亡的影响。方法选取30只免疫缺陷的BALB/c小鼠和30只C57BL/6小鼠,随机分为3组,每组两种小鼠各10只,分别为对照组、移植组和白介素-2组。移植组将C57BL/6小鼠肾脏移植给BALB/c小鼠24 h后将正常的MSCs静脉注入到移植受体中,白介素-2组将IL-2 mRNA及目的基因导入MSCs中并静脉注入到移植受体中;检测小鼠体内IL-2 mRNA及IL-2R mRNA表达情况,ELISA法检测小鼠血液中IL-2、TGF-β1、sFas及其配体sFasL水平;Western blot检测小鼠肾小管上皮细胞JAK、STAT3、p-STAT3表达。结果相比对照组,移植组IL-2 mRNA、IL-2R mRNA表达,血清TGF-β1、sFas及sFasL水平,小鼠肾小管上皮细胞JAK和p-STAT3蛋白表达升高,且差异有统计学意义(P<0.05),肾小管上皮细胞STAT3表达差异无统计学意义(P>0.05);相比移植组,白介素-2组小鼠IL-2 mRNA、IL-2R mRNA表达,血清TGF-β1、sFas及sFasL水平,小鼠肾小管上皮细胞JAK和p-STAT3表达升高,且差异有统计学意义(P<0.05),肾小管上皮细胞STAT3表达差异无统计学意义(P>0.05)。结论白介素-2可以通过促进Fas/FasL的表达介导T细胞凋亡增强对移植肾脏排斥。
Objective To explore effects of interleukin-2(IL-2)and IL-2 receptor(IL-2R)on Fas/FasL expression and T cell apoptosis in renal transplantation immune tolerance induced by bone marrow mesenchymal stem cells(MSCs).Methods Thirty BALB/c mice with immunodeficiency and thirty C57BL/6 mice were randomly divided into 3 groups(control group,transplant group,IL-2 group),10 the former mice and 10 the latter mice in each group.In transplant group,kidneys of C57BL/6 mice were transplanted into BALB/c mice.After 24 h,normal MSCs were intravenously injected into transplant receptors.In IL-2 group,IL-2 mRNA and target gene were introduced into MSCs,and then they were intravenously injected into transplant receptors.The expression of IL-2 mRNA and IL-2R mRNA in mice was detected.The levels of IL-2,transforming growth factor β1(TGF-β1),soluble apoptosis-related factors(sFas)and their ligands(sFasL)in mice blood were detected by ELISA.Western blot was applied to detect the expression of JAK,STAT3 and p-STAT3 in renal tubular epithelial cells.Results Compared with control group,the expression of IL-2 mRNA and IL-2R mRNA,levels of serum TGF-β1,sFas and sFasL,and the expression of JAK and p-STAT3 proteins in renal tubular epithelial cells were increased in transplant group(P<0.05).There was no statistical difference in STAT3 in renal tubular epithelial cells(P>0.05).Compared with transplant group,the expression of IL-2 mRNA and IL-2R mRNA,levels of serum TGF-β1,sFas and sFasL,and the expression of JAK and p-STAT3 proteins in renal tubular epithelial cells were increased in in IL-2 group(P<0.05).There was no statistical difference in STAT3 in renal tubular epithelial cells(P>0.05).Conclusion Il-2 can enhance rejection to transplanted kidney by promoting the expression of Fas/FasL and mediating apoptosis of T cells.
作者
曹荣华
石小红
Cao Ronghua;Shi Xiaohong(Dept of Organ Transplantation,The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510016;Dept of Doppler Ultrasonic,The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510016)
出处
《安徽医科大学学报》
CAS
北大核心
2020年第5期717-721,共5页
Acta Universitatis Medicinalis Anhui
基金
广东省科技计划重点项目(编号:2015B020226005)。
关键词
肾移植
免疫耐受
白介素-2
间充质干细胞
排斥
kidney transplant
immune tolerance
interleukin-2
mesenchymal stem cells
rejection
