摘要
目的:比较子芩与枯芩对大鼠大肠湿热证的药效差异,通过现代药理实验验证将黄芩分为子芩与枯芩分别入药的科学性。方法:将大鼠随机分为空白组,模型组,子芩低、高剂量组(0.9,3.6 g·kg-1),黄芩组(0.9 g·kg-1),枯芩低、高剂量组(0.9,3.6 g·kg-1),复方黄连素片组(0.045 g·kg-1),每组8只。选择大肠湿热证大鼠模型,选择体温、胸腺指数、脾脏指数、回肠病理切片、结肠病理切片、炎症因子以及分泌型免疫球蛋白(SIg)A含量为指标评价子芩与枯芩对大肠湿热证的治疗作用,综合评价子芩与枯芩的药效差异。采用偏最小二乘法-判别分析(PLS-DA)对2种饮片抗大肠湿热证药理指标进行统计学分析。结果:不同剂量的子芩和枯芩均能降低大肠湿热证模型大鼠的体温、胸腺指数和脾脏指数、血清炎症因子白细胞介素(IL)-2,IL-6,IL-1β和肠黏膜SIg A含量,且大部分具有显著性差异(P<0.05,P<0.01);与等剂量枯芩组比较,相应剂量子芩组效果更好,且大部分具有显著性差异(P<0.05,P<0.01)。PLS-DA结果显示子芩与枯芩各聚类于一侧。结论:子芩与枯芩对大肠湿热证均具有治疗作用,但两者的作用强度有明显差异,子芩优于枯芩,验证了古方中用子芩专泻大小肠下焦之火的科学性。
Objective:To compare the pharmacodynamic effects of pith-nodecayed and pith-decayed products of Scutellariae Radix on rats with large intestine damp-heat syndrome,and to demonstrate the scientificness of dividing Scutellariae Radix into pith-nodecayed and pith-decayed products as medicines by modern pharmacological test.Method:Rats were randomly divided into blank group,model group,low-and high-dose group of pith-nodecayed products(0.9,3.6 g·kg-1),low-and high-dose group of pith-decayed products(0.9,3.6 g·kg-1),Scutellariae Radix group(0.9 g·kg-1),compound berberine tablets group(positive drug group,0.045 g·kg-1),and 8 rats in each group.Taking model rats with large intestine damp-heat syndrome,the body temperature,thymus index,spleen index,pathological sections of colon and ileum,inflammatory factors and Secretory immunoglobulin(SIg)A content were selected as indexes to evaluate the therapeutic effect of pithnodecayed and pith-decayed products on large intestine damp-heat syndrome,and make comprehensive evaluation of the difference in efficacy between them.Partial least squares-discriminant analysis(PLS-DA)was employed to analyze the pharmacological indexes of these two products against large intestine damp-heat syndrome.Result:Pith-nodecayed and pith-decayed products of Scutellariae Radix with different doses could reduce the body temperature,thymus index,spleen index,contents of interleukin(IL)-2,IL-6,IL-1βin serum and SIgA content in intestinal mucosa,and most of them had significant differences(P<0.05,P<0.01).Compared with the isodose group of pith-decayed products,the effect of corresponding dose group of pith-nodecayed products was better,and most of them had significant differences(P<0.05,P<0.01).PLS-DA results indicated that there were significant differences in the pharmacological effects of pith-nodecayed and pith-decayed products,and they were clustered on one side,respectively.Conclusion:Both of pith-nodecayed and pith-decayed products of Scutellariae Radix have therapeutic effect on large intestine damp-heat syndrome with distinctly different strength of action,and pith-nodecayed products is superior to pith-decayed products,which verify the scientific nature of pithnodecayed products was specializedly used to treat bowel disease in ancient times.
作者
张方蕾
赵佳文
胡江慧
肖锟钰
谢颖
雷林
刘艳菊
ZHANG Fang-lei;ZHAO Jia-wen;HU Jiang-hui;XIAO Kun-yu;XIE Ying;LEI Lin;LIU Yan-ju(Engineering Research Center of Processing Technology of Traditional Chinese Medicine in Hubei Province,School of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2020年第7期57-64,共8页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技部基础性工作专项(2014FY111100-2)
国家中医药管理局2015中药炮制技术传承基地建设项目(国中医药规财发[2015]21号).
关键词
黄芩
子芩
枯芩
大肠湿热证
偏最小二乘法-判别分析
炎症因子
分泌型免疫球蛋白A
Scutellariae Radix
pith-nodecayed products of Scutellariae Radix
pith-decayed products of Scutellariae Radix
large intestine damp-heat syndrome
partial least squares-discriminant analysis
inflammatory factors
secretory immunoglobulin A
