摘要
为明确铁皮石斛根腐病病原真菌,于其主产地浙江省金华市武义县收集铁皮石斛根腐病病株,采用平板分离方法对病原真菌进行分离,使用镰刀菌种特异性引物并结合ITS和TEF序列分析及形态学鉴定确定该镰刀菌的分类地位。结果表明,共分离纯获得真菌117株,其中有105株镰刀菌;经分子生物学分析及形态学鉴定结果显示,分离出的镰刀菌为层出镰刀菌Fusarium prolife‐mum、茄病镰刀菌F. solani、尖孢镰刀菌F. oxysporum和厚垣镰刀菌F. chlamydosporum四个种,其中层出镰刀菌在数量上具有优势地位,占总镰刀菌数的44.8%;茄病镰刀菌、尖孢镰刀菌、厚垣镰刀菌分别占总镰刀菌数的21.0%、15.2%和19.0%。在致病性测定中发现层出镰刀菌和茄病镰刀菌并不具备致病性,尖孢镰刀菌的致病性明显弱于厚垣镰刀菌,表明厚垣镰刀菌为浙江省金华市武义县铁皮石斛根腐病的主要致病菌。
In order to clarify the pathogenic fungi of root rot of traditional medicinal herb Dendrobium officinale,some diseased plants of D.officinale were collected from Wuyi County,Jinghua City,Zhejiang Province,the main production area of D.officinale.One hundred and seventeen fungal strains were obtained by plate isolation and purification and identified by PCR using the Fusarium specific primer pairs in combination with ITS,TEF sequence analysis and morphological method.The results showed that 105 strains belonged to four different species,i.e.,F.prolifemum,F.solani,F.oxysporum and F.chlamydosporum.F.prolifemum was the predominant species with a percentage of 44.8%.The percentages of F.solani,F.oxysporum and F.chlamydosporum were 21.0%,15.2% and 19.0%,respectively.It was found that F.prolifemum and F.solani had no pathogenicity against D.officinale in the pathogenicity test.Moreover,the pathogenicity of F.oxysporum on D.officinale was obviously weaker than that of F.chlamydosporum.It suggested that F.chlamydosporum was the main pathogen of D.officinale root rot in Wuyi County,Jinghua City,Zhejiang Province.
作者
曹瑱艳
杨怡华
申屠旭萍
俞晓平
CAO Zhenyan;YANG Yihua;SHENTU Xuping;YU Xiaoping(Zhejiang Provincial Key Laboratory of Biometrology and Inspection&Quarantine,Chi na Jiliang University,Hangzhou 310018,Zhejiang Province,China)
出处
《植物保护学报》
CAS
CSCD
北大核心
2020年第1期178-186,共9页
Journal of Plant Protection
基金
浙江省重点研发计划项目(2018C02030,2019C02015)
杭州市科技计划项目(20190101A01)
关键词
铁皮石斛
根腐病
镰刀菌
病原真菌鉴定
Dendrobium officinale
root rot
Fusarium spp.
identification of pathogenic fungi
