摘要
目的建立动物皮肤感染脓肿模型评价指标体系,筛选优质金黄色葡萄球菌感染小鼠皮肤脓肿模型,并通过多枝雾水葛抗皮肤感染药效评价验证模型。方法在调研文献和咨询专家的基础上,利用层次分析法建立皮肤感染脓肿模型的综合评价指标体系,将小鼠分成4组,包括对照组,金黄色葡萄球菌高、中、低浓度组,观察记录19 d,综合评价小鼠皮肤脓肿模型;将筛选出的最佳小鼠皮肤感染脓肿模型运用于多枝雾水葛抗皮肤感染药效评价。结果所构建的动物皮肤感染脓肿模型评价指标体系比较全面,指标权重增强了评价的客观性;小鼠皮下注射1×10^13 cfu·L^-1金黄色葡萄球菌菌液0.1 mL建立的脓肿模型成功率高、稳定性好,综合评分最高;多枝雾水葛软膏可有效促进脓肿消退,药物组脓肿体积明显减小,皮肤细菌含量明显降低,炎症反应减轻。结论该实验成功构建了动物皮肤感染脓肿模型评价指标体系,优选的小鼠皮肤脓肿模型为进一步研究皮肤感染疾病和治疗药物提供了可靠的动物模型,多枝雾水葛软膏具有抗皮肤感染作用。
Objective To establish the evaluation index to system of animal skin infection abscess model,to screen the high-quality model of skin abscess in mice infected with Staphylococcus aureus,and to verify the model by evaluating the efficacy of Pouzolzia zeylanica. Methods On the basis of literature research and expert consultation,to the comprehensive evaluation index system of skin infection abscess model was established by the analytic hierarchy process.The mice were randomly divided into four groups, including the control group and the high, medium and low concentration groups of staphylococcus aureus.All groups were observed and recorded for 19 days, then the models were comprehensively evaluated.The best model was selected and applied to evaluate the anti-skin infection efficacy of Pouzolzia zeylanica. Results The evaluation index system of animal skin infection abscess model was relatively comprehensive, and the index weight enhanced the objectivity of evaluation.The abscess model in mice which established by subcutaneous injection with 0.1 mL staphylococcus aureus solution(1×10^13 cfu·L^-1)had high success rate and good stability,and it had the highest overall score.Abscess and inflammation and bacterial infection significantly decreased with the increasing dose of Pouzolzia zeylanica ointment. Conclusion The evaluation index system of animal skin infection abscess model is successfully constructed.The optimal skin mice model infected with staphylococcus aureus provides a reliable animal model for study of skin infections and therapeutic drugs.Pouzolzia zeylanica ointment has anti-skin infection effect.
作者
江炜
邹国发
古炽明
陶曙红
郭丽冰
唐春萍
杨超燕
陈艳芬
JIANG Wei;ZOU Guofa;GU Chiming;TAO Shuhong;GUO Libing;TANG Chunping;YANG Chaoyan;CHEN Yanfen(School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China;NO.2 Surgical Department of Guangdong Provincial Hospital of TCM,Guangzhou 510105,China;Guangzhou Key Laboratory of Construction and Application of New Drug Screening Model Systems,Guangzhou 510006,China)
出处
《医药导报》
CAS
北大核心
2020年第3期298-304,共7页
Herald of Medicine
基金
广东省公益研究与能力建设专项资金项目(2015A030302088)
广东省科技厅疾病防治项目(2016A020215158)
广东省科技厅-广东省中医药科学院联合项目(2014A020221095)。
关键词
多枝雾水葛软膏
皮肤脓肿模型
层次分析法
金黄色葡萄球菌
Pouzolzia zeylanica ointment
Skin abscess model
Analytic hierarchy process
Staphylococcus aureu
