摘要
绵马贯众是中国传统常用中药,本研究以温度、时间、超声功率、液料比为影响因子,多糖得率为评价指标,通过响应面法优化超声辅助提取绵马贯众多糖的工艺条件,同时测定其基本理化性质及抗氧化活性。研究结果表明,绵马贯众多糖的最佳提取工艺条件为:温度64℃、时间60 min、超声功率210 W、液料比27 mL/g。此时多糖得率为9.57%,与预测值接近。理化性质分析表明绵马贯众多糖为含少量蛋白的酸性多糖。体外抗氧化研究表明绵马贯众多糖具有很强的DPPH自由基清除活性,IC50值为0.29 mg/mL;较好的羟基自由基清除活性,其IC50值为1.10 mg/mL;对DNA的氧化损伤有显著的保护作用。绵马贯众多糖可以作为一种潜在的抗氧化剂应用于食品和化妆品等领域。
Male Fern Rhizome is a kind of traditional Chinese medicine.This research was aimed to explore the optimalizing conditions of ultrasound-assisted extraction polysaccharides from Dryopteris crassirhizoma Nakai.Response surface method was employed to maximize extraction yield through the temperature,time,ultrasonic power,and water-material ratio.The basic physical and chemical properties and antioxidant activity of the Dryopteris crassirhizoma polysaccharides were also measured.The results showed that the optimal extraction conditions were temperature of 64℃,time of 60 min,ultrasonic power of 210 W,and water-material of 27 m L/g.Under these conditions,the actual extraction yield was 9.57%,which was quite close to the predicted value.And the polysaccharides were acid polysaccharides with a little protein.The Dryopteris crassirhizoma polysaccharides exhibited strong antioxidant abilities to scavenge DPPH radical and its IC50 was 0.29 mg/mL.And these polysaccharides also showed high activity of hydroxyl free radicals with 1.10 mg/mL of IC50.Furthermore,Dryopteris crassirhizoma polysaccharides showed strong protection against DNA oxidative damage.Therefore,Dryopteris crassirhizoma polysaccharides might be used as a potential antioxidant in food and cosmetics industry.
作者
李佳颖
高誉
刘红
肖羽
万岚凤
赵雨晴
胡超
袁明
Li Jiaying;Gao Yu;Liu Hong;Xiao Yu;Wan Lanfeng;Zhao Yuqing;Hu Chao;Yuan Ming(College of Life Sciences,Sichuan Agricultural University,Ya'an,625014)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2020年第1期79-87,共9页
Genomics and Applied Biology
基金
四川省科技厅科技支撑计划苗子工程重大项目
四川省大学生创新创业训练计划(201710626084)
四川农业大学大学生创新训练计划项目(201810626050)共同资助。
关键词
绵马贯众
多糖
超声辅助提取
响应面
抗氧化活性
Male Fern Rhizome
Polysaccharides
Ultrasonic assisted extraction
Response surface analysis
Antioxidant activity
