摘要
用超临界流体萃取法（ｓｕｐｅｒｃｒｉｔｉｃａｌｆｌｕｉｄｅｘｔｒａｃｔｉｏｎ，ＳＦＥ）代替传统的溶剂萃取法对中药材马蓝（Ｓｔｒｏｂｉｌａｎｔｈｅｓｃｕｓｉａ）、菘蓝（Ｉｓａｔｉｓｔｉｎｃｔｏｒｉａ）和蓼蓝（Ｐｏｌｙｇｏｎｕｍｔｉｎｃｔｏｒｉｕｍ）中有效成分靛玉红（ｉｎｄｉｒｕｂｉｎ）的萃取条件进行了研究，并用微孔高效液相色谱法做了含量监测。结果证明：ＳＦＥ省时、省力、经济、选择性可调的性能很强，与传统的溶剂萃取法相比，有独特的优点。
This study indicates that supercritical fluid extraction ( SFE ) can
be adopted jnstead of traditional solvent extraction to extract
the active constituent indirubin in Chinese medicinal
materials(Strobilanthes cusia,Isatis tinctoria and Polygonum
tinctorium).A two-level , two- factor(22) factotial design approach
is used to optimize the temperature and pressure in SFE.A systematic
method is also used to optimize five variables(temperature ,
pressure , modifier concentration , static extracting time and CO2
dynamic extracting volume)of SFE. The results demonstrate the utility
and efficiency of factorial design in two-level ,
two-factor(22)for optimizing SFE temperature and pressure , and also
show faster extraction kinetics and the ability of SFE to vary the
extracting power through adjustments of the above five
variables.Sample extracts were analyzed bymicrobore high performance
liquid chromatography(MHPLC).The method showed good
linearity(r=0.9998,n=5)in the range of 2.11～10.57 μg·ml-1 of
indirubin.The average recovery was 94. 63%with RSD<0. 92%(n=4). MHPLC
condition : column : Spherisorb 3μm,C18( 1 mm × 1 5 0 mm);mobile
phase : methanol-water(7:3);flow rate : 40μl·min-1;UV detection :
290 nm;recorder sensitivity: 0. 01 AUFS; injection volume : 0.1μl.
出处
《药学学报》
CAS
CSCD
北大核心
1995年第2期133-137,共5页
Acta Pharmaceutica Sinica
关键词
超临界流体萃取
高效液相色谱
中药材
Supercritical fluid extraction
Strobilanthes cusia
Isatis
tinctoria
Polygonum tinctotrium
Indirubin
Microbore high
performance liquid chromatography
