摘要
目的研究中药贯众(DCN)提取物对体外培养的人肝癌细胞的抑癌活性。方法 DCN经粉碎、浸提、超速离心、减压浓缩、冷冻干燥得DCN提取物。人肝癌SMMC-7721细胞经DCN提取物处理不同时间后以MTT法测其对人肝癌细胞线粒体代谢活性的影响,以细胞计数法观察了其对细胞增殖的影响。结果(1)DCN提取物能明显抑制人肝癌细胞的生长,降低线粒体代谢活性;(2)经DCN提取物处理24h后的人肝癌细胞,既使更换为正常培养基,其线粒体代谢活性及细胞增殖与对照相比仍差异显著。结论中药DCN提取物含抑癌活性物质,能抑制体外培养的肝癌细胞增殖和降低线粒体代谢活性。
Objective To demostrate the effects of the extracts from traditional Chinese drug Dryopteris cras-sirhizoma Nakai (DCN) on the proliferation and mitochondria metabolism of human hepatoma SMMC^7721 cells. Methods The dried DCN was broken to powder, extracted in cold DW and centrifuged to discard the pellet and the DCN supernatant was concentrated and lyophilized. Human hepatoma cells were treated with 50ug/ml DCN extracts for 48h and mitochondria metabolism activity was detected by MTT. After treated with DCN extracts for 24h and then replaced by cultured in normal media (containing 10% PCS) for 96h. Results DCN extracts could were treated for 24h and then replaced by cultured in normal media for 96h, the cells entered immedeately into logarithmic phase after the drug was removed from the medium. Conclusion DCN extracts could inhibit the proliferation and mitochondria metabolism activity of human hepatoma cells.
出处
《医学研究通讯》
2000年第5期5-7,共3页
Bulletin of Medical Research
基金
山东省教委资助(J97K01)
关键词
细胞增殖
线粒体
中药
肝肿瘤
贯众水提物
Hepatoma SMMC-7721 Dryopteris crassirhizoma Nakai DCN proliferation Mitochondria MTT
