摘要
目的 研究大黄素对血管紧张素Ⅱ (AngⅡ )刺激人肾成纤维细胞 (KFB)增殖、胶原表达等的抑制效应。方法 用3 H TdR掺入法 ,流式细胞仪及免疫组织化学技术观察了AngⅡ对KFB增殖 ,细胞周期及Ⅰ型胶原表达的影响以及大黄素对AngⅡ作用于KFB的保护效应。结果 ①AngⅡ 10 -10 mol/L至 10 -6mol/L增加KFB3 H TdR掺入率 ,第 1、3天呈计量依赖关系 (r1=0 .70 9,P <0 .0 1;r3 =0 .80 6,P <0 .0 1) ;不同浓度的大黄素 (3 0～ 10 0 μg/ml)第 1、3和 5抑制了AngⅡ 10 -6mol/L诱导的KFB3 H TdR掺入率 ,呈剂量依赖性特点 (r = 0 .890 , 0 .947和 -0 .891;P <0 .0 1) ;而且大黄素本身也可抑制KFB3 H TdR掺入率 ,呈剂量依赖性特点。②AngⅡ在 10 -6mol/L明显促进KFBG1向S期转化 (P <0 .0 1) ;大黄素在 10 0 μg/ml抑制了KFBG1向S期转化 (P <0 .0 1)。③AngⅡ在 10 -6mol/L增加了KFBⅠ型胶原表达 (P <0 .0 5 ) ,大黄素明显降低了AngⅡ诱导的Ⅰ型胶原表达 (P <0 .0 5 )。结论 大黄素可抑制AngⅡ诱导的KFB增殖 ,Ⅰ型胶原表达 。
Objective To study the inhibitive effect of Emodin on the proliferation of human kidney fibroblasts(KFB)and the expression of collagen type Ⅰ on FKB stimulated by angiotensin Ⅱ.Methods We observed the effect of Ang Ⅱ on KFB proliferation,cell cycle and the expression of collagen type Ⅰ on FKB,as well as the inhibiting effect of Emodin with 3H TdR incorporation method,Flowcytometry and immunohistochemical technique respectively.Results Exposure of KFB to Ang Ⅱ (10 -10 ～10 -6 mol/L)induced dose dependent increase in 3H TdR corporation at 1st and 3rd days(r=0.709,0.806, P <0.01).AngⅡ(10 -6 mol/L)could promote significantly the progression of KFB G 1 to S phase( P <0.01) and increase the expression of collagen type Ⅰ( P <0.05).The rising KFB 3H-TdR incorporation induced by Ang Ⅱ (10 -6 mol/L) Could be inhibited by Emodin with diflerent concentration ( P <0.01).Emodin(100μg/ml)Could not only delay the progress of KFB G 1 to S phase,but also reduce the expression of collagen type Ⅰ.Conclusion The emodin can inhibit proliferation of KFB and the expression of collagen type Ⅰ induced by Ang Ⅱ.Therefore,The emodin may play an important role in prevention of renal interstitial fibrosis.
出处
《四川医学》
CAS
2002年第11期1114-1117,共4页
Sichuan Medical Journal
