摘要
目的观察补肾生血颗粒剂对肾小管上皮细胞和肾成纤维细胞共培养体系产生细胞外基质的影响,并探讨其机理。方法采用血清药理学方法,细胞生物学方法研究共培养的成纤维细胞的增殖及凋亡;用 ELISA 法测定肾小管上皮细胞和肾成纤维细胞共培养体系中细胞外基质的含量。结果补肾生血颗粒剂各剂量组能明显抑制共培养的成纤维细胞增殖,促进其凋亡,减少共培养体系中纤维连接蛋白(FN)、Ⅳ型胶原的含量。结论补肾生血颗粒剂能明显减少肾小管上皮细胞和肾成纤维细胞共培养体系中细胞外基质的含量,其机制可能与抑制成纤维细胞增殖,促进其凋亡有关。
Objective To observe the effect of Bushen Shengxue Granule(BSG)on extracellular matrix induced by co-cul- tured renal tubular endothelial cell and renal fibroblast,and to explore its mechanism.Methods Serum pharmacological method was adopted.The proliferation and apoptosis of co-cultured fibroblast was determined by cellular biological method;content of extracellular matrix in the co-culture system of renal tubular endothelial cell and renal fibroblast de- termined by ELISA method.Results All the dosages of...
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2004年第S1期128-131,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
上海市卫生局课题资助(No.01-44)
