摘要
目的 :观察血管紧张素Ⅱ (AngⅡ )对人肾成纤维细胞 (KFB)的影响。 方法 :采用ELISA法、四甲基偶氮唑蓝 (MTT)法、流式细胞仪、免疫组织化学法分别检测AngⅡ对KFB分泌的胶原酶活性、KFB增生、凋亡、Ⅰ型胶原表达的影响。 结果 :AngⅡ能提高KFB分泌的总胶原酶活性、促进KFB增生、抑制KFB凋亡、促进KFBⅠ型胶原的表达。 结论 :AngⅡ能通过促进KFB增生、抑制KFB凋亡、促进KFBⅠ型胶原的表达来促进肾间质纤维化。
To study the effects of angiotensing Ⅱ on human kidney fibroblasts(KFB)in its secreted collagenases activity,proliferation,apoptosis and expression of type Ⅰ collagen. METHODOLOGY The activity of type Ⅰ collagenase,KFB apoptosis,proliferation and collagen Ⅰ expression were detected by ELISA,flow cytometer,MTT and immunohistochemical method respectively. RESULTS Angiotensin Ⅱ enhanced the activity of collagenase secredted by KFB,inhibited KFBs apoptosis,promoted the KFBs proliferation and enhanced the expression of type Ⅰ collagen in KFB. CONCLUSION Angiotensin Ⅱ can induce renal fibrosis by inhibiting apoptosis,promoting proliferation and expression of type Ⅰ collagen of KFB.
出处
《肾脏病与透析肾移植杂志》
CAS
CSCD
2000年第1期30-33,共4页
Chinese Journal of Nephrology,Dialysis & Transplantation
