摘要
为进一步探讨运动心脏重塑的发生机制 ,本研究采用反转录聚合酶链式反应 (RT -PCR)技术 ,对 60只SD大鼠 1 2周耐力训练过程中心肌胰岛素样生长因子 (IGF -Ⅰ )的表达进行了观察。结果表明 ,与安静对照组相比 ,耐力训练过程中心肌组织中IGF -Ⅰ在转录水平的表达均显著增强 ,其中 ,心房IGF-ⅠmRNA的表达在训练 1 0天达到高峰 (P <0 0 1 ) ,心室IGF -ⅠmRNA的表达在训练 3天即达到高峰 (P <0 0 1 ) ,训练 40天后心房、心室IGF -ⅠmRNA的表达基本恢复到正常对照水平。结果提示 ,心血管调节肽IGF -Ⅰ在运动性心脏重塑发生过程中起上调作用 ,且这种上调作用在运动心脏发生的早期即已启动 ,心房和心室组织中IGF
In order to approach the developing mechanism of cardiac remodeling induced by exercise, sixty male SD rats were randomly divided into two groups, controls and training with moderate intensity (70%～80%VO 2max) on a treadmill, and their expression of cardiac insulin-like growth factors (IGF-I) in the processes of endurance training were investigated by reverse transcription polymerase chain reaction technique(RT-PCR).The results showed that the level of myocardial IGF-Ⅰ mRNA in trained rats were significantly higher than their controls in different training periods, respectively. The expression of atria IGF-Ⅰ mRNA appeared as early as 3rd day and reached maximal level on 10th day, but the maximum mRNA level for ventricular IGF-Ⅰ increased at 3rd day. Those changes maintained for 40 days. The results suggested that IGF-Ⅰ played an up-regulation roles in the processes of exercise induced cardiac remodeling and those up-regulation roles started at early stage of exercise-induced cardiac hypertrophy. Also, there were different regulating time causes and regulating ways between atria and ventricle.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2002年第6期565-569,共5页
Chinese Journal of Sports Medicine
